不同日龄苏太仔猪LTβR基因组织表达谱及发育性表达规律分析

Tissue Expression Profile and Differential Expression of LTβR Gene in Sutai Piglets of Different Ages

试验旨在探讨LTβR基因在仔猪出生至断奶期间的mRNA表达变化,为进一步研究该基因与F18大肠杆菌致病的相关性提供理论依据。本试验选取从初生到断奶的4个日龄(8、18、30和35日龄)苏太仔猪各4头,利用实时荧光定量PCR方法分别比较分析了LTβR基因在各个体11个组织(心脏、肝脏、脾脏、肺脏、肾脏、胃、肌肉、胸腺、淋巴结、十二指肠及空肠)间的表达规律。结果表明,LTβR基因在肝脏、脾脏、肺脏、肾脏、胃、淋巴结、十二指肠及空肠组织中呈现较高水平的表达,并且表现出明显的发育性表达差异。LTβR基因在8日龄仔猪淋巴、十二指肠和空肠组织中的表达极显著高于其他日龄(P<0.01);在8日龄仔猪肺脏组织中的表达极显著高于35日龄(P<0.01),且显著高于30日龄(P<0.05);在35日龄仔猪肝脏组织中的表达极显著高于其他日龄(P<0.01);在35日龄仔猪胃组织中的表达显著高于18日龄(P<0.05)。由此推测,8日龄左右为仔猪肠道免疫屏障快速形成期,LTβR基因的较高表达可能有利于仔猪对F18大肠杆菌抗性。

Dynamic changes of LTβR expression levels in 11tissues（heart,liver,spleen,lung,kidney,stomach,muscle,thymus,lymph node,duodenum and jejunum）of Sutai piglets ranging from newborn to post-weaning days 8,18,30,and 35 were compared and analyzed by the Real-time PCR method,which aimed to provide theoretical basis for further investigate the relationship between LTβRgene and pathogenicity of E.coli F18.The results revealed that the LTβR expression levels were higher in the liver,spleen,lung,kidney,stomach,lymph node,duodenum and jejunum,and showed obvious age-dependent expression differentiation.The LTβR expression levels in the lymph node,duodenum,and jejunum were extremely significant higher in 8days old piglets than in the other age stages（P〈0.01）,and the expression levels were extremely significantly higher in the lungs of 8days old piglets than in 35 days old piglets（P〈0.01）and significantly higher than 30 days old piglets（P〈0.05）.In the liver tissue,the expression level was extremely significant higher in 35 days old piglets than in other age stages（P〈0.01）.In the stomach tissue,theexpression level was significantly higher in 35 days old piglets than in 18 days old piglets（P〈0.05）.The results speculated that intestinal immune barrier of piglets formed rapidly around8 days old and the higher LTβR expression could contribute to the resistance to E.coli F18.