反复右美托咪定联合丙泊酚麻醉对成年大鼠学习记忆能力的影响

Impact of Repeated Use of Dexmedetomidine and Propofol on the Ability of Learning and Memory in Adult Rats

目的观察反复右美托咪定联合丙泊酚麻醉对成年大鼠学习记忆能力的影响。方法选取8周龄SD大鼠40只,雌雄不分,随机分为四组各10只:丙泊酚组(P组),右美托咪定组(D组),右美托咪定联合丙泊酚组(DP组),生理盐水组(NS组)。P组给予30 mg/kg的丙泊酚,D组给予30μg/kg的右美托咪定,DP组给予30 mg/kg的丙泊酚和30μg/kg的右美托咪定,NS组给予等量生理盐水,所有药物均采用尾静脉注射法,每日1次,连续5天,第6天起,选用Morris水迷宫进行行为学实验。结果在定位航行实验中,D组、DP组、NS组与P组比较,前4天的潜伏期差异有统计学意义(P<0.05),其中P组的潜伏期均明显长于DP组、D组、NS组(P<0.05);DP组、NS组与D组比较,前4天的潜伏期差异无统计学意义(P>0.05),且第2、3、4天三组的潜伏期均明显短于第1天(P<0.05);在空间探索实验中,各组间各项数据差异均无统计学意义(P>0.05)。结论右美托咪定对于在镇静剂量下丙泊酚麻醉所导致的成年大鼠的学习记忆功能的短暂损伤具有保护作用。

Objective To observe the impact of repeated use of dexmedetomidine （Dex） and propofol on the ability of learning and memory in adult rats. Methods Forty 8-week-old SD rats were randomly divided into four groups （n = 10）： propofol group （group P）, Dex group （group D）, Dex combined with propofol group （group DP） and normal saline group （group NS）. Group P received 30 mg/kg of propofol, group D received 30/xg/kg of Dex, group DP received 30 mg/kg of propofol and 30 ~g/kg of Dex, group NS was given the same amount of NS, all drugs were injected by caudal vein, once daily for 5 consecutive days, then the ability of learning and memory were tasted after Morris water maze experiments. Results In place navigation test, the difference of the first four days of latency between group P and group D, group DP, group NS was statistical （P〈0.05）, the latency period of group P was significantly longer than that of group DP, group D, group NS （P〈0.05）; the difference of the first four days of latency between group D and group DP, group NS was not significant （P〉0.05）, and the latency period in the first day of three groups was longer than that in the second day, third day and fourth day （P〈0.05）. In the space exploration test, the difference among each group was not statistical （P〉0.05）. Conclusions Dexmedetomidine has protective effect on the brief injury of learning and memory ability resulting from anesthesia with propofol under sedation dose in adult rats.