P物质对表皮细胞聚集、分化控制及在皮肤创伤修复中的应用

Substance P effects on aggregation and differentiation control of epidermal stem cells and skin wound repair

背景:表皮干细胞是皮肤及其附属器修复的源泉细胞,研究其分化、迁移的调控机制是实现其完美愈合的关键。目的:探讨P物质在皮肤创伤修复中的应用效果。方法:采用随机对照方法将30只Wistar大鼠分为P物质组、辣椒素组和对照组,每组10只。建立皮肤缺损模型后分别于创面局部注射外源性神经肽P物质、辣椒素、生理盐水,均连续注射5 d。用药不同时间点检测3组大鼠皮肤厚度、皮肤创伤面积、BrdU阳性细胞率。结果与结论:1用药第14天3组大鼠皮肤厚度差异无显著性意义(P>0.05);随着用药时间的不断延长,表皮厚度开始变薄;用药第42天,P物质组表皮厚度明显缩小,对照组表皮厚度变化不明显,辣椒素组表皮厚度有所缩小。2皮肤创伤3,5,7 d时P物质组皮肤创伤面积显著小于对照组和辣椒素组(P<0.05),辣椒素组和对照组的创伤面积差异无显著性意义(P>0.05)。3P物质组在用药第3,5,7,12天时Brd U细胞阳性率显著高于辣椒素组和对照组(P<0.05);辣椒素组在第3,5,7,12天时BrdU细胞阳性率显著低于对照组(P<0.05)。4结果提示P物质能够促进表皮细胞增殖,加速创面部位愈合。

BACKGROUND： Epidermal stem cells are the source of cells for repairing the skin and its appendages. To study differentiation and migration of epidermal stem cells is crucial for perfect healing. Objective： To investigate the effects of substance P in skin wound repair.METHODS： Thirty Wistar rats were randomized into groups of substance P, capsaicin and control（n=10 per group）. After the establishment of skin defect models, exogenous neuropeptide substance P, capsaicin, and normal saline were daily injected at the wound for continuous 5 days. Skin thickness, skin wound area, BrdU-positive cells were detected in the three groups at different time after injection. Results and conclusions： No difference n the skin thickness was found in the three groups at 14 days after injection（P〉0.05）, but with time, the epidermal thickness was reduced. At 42 days, the epidermal thickness was reduced remarkably in the substance P group and decreased certainly in the capsaicin group, but unchanged in the control group. The wound area of the substance P group was significantly lower than that in the control and capsaicin groups at 3, 5, 7 days after injection（P〈0.05）, but there was no difference between the control and capsaicin group（P〉0.05）. The Brd U positive rates in the three groups were significantly different at 3, 5, 7, 12 days after injection： the substance P group the control group the capsaicin group（P〈0.05）. These results show that substance P can promote epidermal cell proliferation and accelerate wound healing.