聚羟基丁酸酯-羟基戊酸酯/溶胶-凝胶生物活性玻璃促进牙周组织再生

Poly(hydroxybutyrate-co-hydroxyvalerate)-sol-gel bioactive glass promotes periodontal tissue regeneration

背景:前期研究证实,聚羟基丁酸酯-羟基戊酸酯/溶胶-凝胶生物活性玻璃具有良好的生物相容性及促骨组织修复作用,但其在牙周组织再生中的作用尚不明确。目的:观察聚羟基丁酸酯-羟基戊酸酯/溶胶-凝胶生物活性玻璃修复比格犬牙周组织缺损的效果。方法:在4只比格犬双侧下颌第三、四前磨牙颊侧制备5 mm×5 mm大小牙槽骨缺损,将左右侧随机分为实验组及对照组,实验组缺损部位置入聚羟基丁酸酯-羟基戊酸酯/溶胶-凝胶生物活性玻璃,对照组缺损部位直接缝合。术后2,4,8,12周进行组织学、扫描电镜、锥形束CT及Ca与P含量比值检测。结果与结论:(1)锥形束CT检测结果:两组新生骨高度均随着时间延长逐渐增加,实验组术后不同时间点的新生骨高度高于对照组(P<0.05)。(2)Ca/P比值检测结果:实验组术后12周的Ca/P比值已接近正常骨组织(P>0.05),且明显高于对照组(P<0.05)。(3)组织学观察结果:术后12周时,实验组新生组织结构接近于正常骨组织,而对照组新生组织趋向成熟,可见少量新生血管。(4)扫描电镜观察结果:术后8周,实验组已不见支架材料结构,可见骨陷窝;对照组无明显成骨细胞及骨陷窝。术后12周,实验组新生组织结构规则,接近正常骨组织结构,未见明显成骨细胞;对照组新生组织排列紊乱,残留部分空腔。(5)结果表明,聚羟基丁酸酯-羟基戊酸酯/溶胶-凝胶生物活性玻璃可有效促进牙周组织再生。

BACKGROUND： Our previous studies have shown that the poly（hydroxybutyrate- co-hydroxyvalerate）-sol-gel bioactive glass（PHBV-SGBG） has good biocompatibility and promote bone tissue repair, but its specific role in periodontal tissue regeneration has not been investigated. OBJECTIVE： To investigate the periodontal regenerative effects of a PHBV-SGBG scaffold in beagle dogs. METHODS： Alveolar bone defects（5 mm×5 mm） were surgically created bilaterally at the buccal side of the mandibular third and fourth premolars of four beagle dogs. PHBV-SGBG scaffold was randomly filled in the defects as experimental group and nothing was put into the contralateral as control group. Histological and scanning electron microscopy observations, cone-beam CT evaluation and the Ca/P concentration ratio analysis were processed at 2, 4, 8 and 12 weeks after surgery. RESULTS AND CONCLUSION： After surgery, the height of the regenerated tissue increased with time in both groups, and the regenerated tissue height in the experiment group was higher than that in the control group（P〈0.05）. At 12 weeks after surgery, the Ca/P concentration ratio of the experiment group was close to that in the normal tissue（P〉0.05）, but higher than that of the control group（P〈0.05）; the histological observation showed that the regenerated tissue of the experimental group was close to the normal tissue, and the regenerated tissue of the control group tended to be mature, with a small amount of new blood vessels. Under the scanning electron microscope, no scaffold structure was visible in the experimental group with the presence of bone lacuna at 8 weeks after surgery, while in the control group, there was no bone lacuna and obvious osteoblasts; at 12 weeks after surgery, the structure of the regenerated tissue of experimental group was more regular and close to the normal tissue with no remarkable osteoblasts, and in the control group, the regenerated tissue was disordered, with several cavity. These results show that the PHBV-SGBG scaffold can enhance periodontal bone regeneration effectively.