左旋聚乳酸羟基磷灰石生物材料与牙周膜细胞的生物相容性

Biocompatibility of poly-L-lactic acid/hydroxyapatite biomaterial with periodontal ligament cells

背景:将羟基磷灰石与左旋聚乳酸复合,可以很好地改善羟基磷灰石质地脆、机械性能差等不足。目的:进一步观察左旋聚乳酸羟基磷灰石生物材料与牙周膜细胞的相容性。方法:将细胞浓度为5×109 L-1的第4代人牙周膜细胞与左旋聚乳酸羟基磷灰石生物材料复合培养,设为实验组,设置单纯牙周膜细胞培养作为对照组。培养7 d内检测两组细胞增殖情况;培养24,48,72 h,检测细胞碱性磷酸活性;培养72 h,检测细胞Ⅰ型胶原表达强度。结果与结论:(1)细胞增殖检测结果:两组培养1-7 d的吸光度值比较差异无显著性意义。(2)细胞碱性磷酸活性检测结果:两组组内培养48,72 h的碱性磷酸酶活性均高于培养24 h(P<0.05),但两组间不同时间点碱性磷酸酶活性比较差异均无显著性意义。(3)细胞Ⅰ型胶原表达检测结果:两组Ⅰ型胶原表达强度比较差异无显著性意义。(4)结果表明,左旋聚乳酸羟基磷灰石生物材料具有良好的细胞相容性。

BACKGROUND： Hydroxyapatite/poly-L-lactic acid composite can well improve the brittleness and mechanical properties of the hydroxyapatite. OBJECTIVE： To study the biocompatibility of hydroxyapatite/poly-L-lactic acid composite biomaterials with periodontal ligament cells. METHODS： Passage 4 human periodontal ligament cells at a density of 5×109/L were cultured with hydroxyapatite/poly-L-lactic acid composite as experimental group, and human periodontal ligament cells cultured alone as control group. Cell proliferation was detected within 7 days of culture; alkaline phosphatase activity was detected at 24, 48 and 72 hours of culture; and type I collagen expression was detected at 72 hours of culture. RESULTS AND CONCLUSION： There was no significant difference in the absorbance value of cells between the two groups at 1-7 days of culture. The alkaline phosphatase activity of cells at 48 and 72 hours was significantly higher than that at 24 hours（P〈0.05） in the two groups, but there was no difference between the two groups. The expression of type I collagen had no difference between the two groups. These findings indicate that the hydroxyapatite/poly-L-lactic acid composite biomaterial has good cell compatibility.