上皮间质转化在胃癌SGC7901细胞向胃癌干细胞转化过程中的作用

Role of epithelial-mesenchymal transition in transformation of gastric cancer cells SGC7901 to gastric cancer stem cells

背景:有研究发现上皮间质转化和肿瘤的侵袭、转移以及耐药性密切相关,但关于上皮间质转化在胃癌SGC7901细胞向胃癌干样细胞转化过程中的作用的研究较为少见。目的:探讨上皮间质转化在胃癌SGC7901细胞向胃癌干样细胞转化过程中的作用。方法:长春新碱诱导胃癌SGC7901细胞,并对长春新碱诱导的胃癌SGC7901细胞进行培养筛选制备胃癌干样细胞,鉴定细胞CD44表型、观察细胞形态变化、检测干细胞相关标志物、检测上皮间质转化相关分子。结果与结论:(1)胃癌SGC7901细胞传代后经过长春新碱诱导后细胞形态发生了改变,无血清悬浮培养后,见克隆样细胞球生成,同时CD44表型的细胞比例下降;(2)胃癌干样细胞中的SOX2和OCT4的表达水平以及间质标志物Snail1 mR NA、Twist mRNA和Vimentin mRNA表达水平均明显高于胃癌SGC7901细胞,但上皮细胞标志物E-钙黏蛋白mRNA表达水平明显低于胃癌SGC7901细胞;(3)结果表明胃癌SGC7901细胞能够成功转化为胃癌干样细胞,上皮间质转化参与了胃癌SGC7901细胞向胃癌干样细胞的转化。

BACKGROUND： Studies have found that epithelial-mesenchymal transition is closely related with tumor invasion, metastasis, and drug resistance, but studies on the role of epithelial-mesenchymal transition in the transformation process of gastric cancer cells SGC7901 to gastric cancer stem-like cells are rarely reported. OBJECTIVE： To explore the effect of epithelial-mesenchymal transition in the transformation process of gastric cancer cells SGC7901 to gastric cancer stem-like cells. METHODS： Vincristine-induced SGC7901 cells were cultured and screened to prepare gastric cancer stem-like cells. CD44 phenotype, morphological changes, stem cell-related markers, and epithelial-mesenchymal transition related molecules were detected. RESULTS AND CONCLUSION： After passage, vincristine-induced SGC7901 cells presented with morphological changes, and clonal cell spheres generated after serum-free suspension culture. Meanwhile, the proportion of SGC7901 cells positive for CD44 was decreased. Expression levels of SOX2,OCT4, Snail1 mRNA, Twist mR NA and Vimentin mRNA were significantly higher in the gastric cancer stem-like cells than SGC7901 cells, but the expression level of E-caderin was lower in the gastric cancer stem-like cells than SGC7901 cells. These findings indicate that gastric cancer cells SGC7901 can be successfully transformed into gastric cancer stem-like cells, and the epithelial-mesenchymal transition is involved in this transforming progress.