瑞舒伐他汀对急性心肌梗死大鼠缺血心肌的影响

Influence of rosuvastatin on rats with acute myocardial infarct

目的观察瑞舒伐他汀对急性心肌梗死大鼠缺血心肌的影响。方法将60只SD大鼠完全随机化分为3组:假手术组(20只),仅给予开、关胸处理,术前1周开始给予生理盐水灌胃,每天1次,持续1周;瑞舒伐他汀组(20只),制备急性心肌梗死模型,术前1周瑞舒伐他汀(5 mg/kg)灌胃,每天1次,持续1周;模型组(20只)处理同瑞舒伐他汀组,但术前1周开始给予等量的生理盐水灌胃,每天1次,持续1周。统计大鼠死亡(术后1 h内死亡)数量;术后8 h取腹主动脉血,采用ELISA法定量检测血清中白介素8(IL-8)、肿瘤坏死因子α(TNF-α)及心肌肌钙蛋白T(c Tn T)的表达情况。结果 60只SD大鼠共存活56只,死亡4只:模型组1只死于呼吸衰竭,2只死于心律失常,瑞舒伐他汀组1只死于麻醉意外。与假手术组比较,模型组和瑞舒伐他汀组大鼠IL-8、TNF-α和c Tn T水平均升高,差异有统计学意义(P均<0.05)。与模型组比较,瑞舒伐他汀组干预后IL-8[(552.91±35.61)μl/ml vs.(264.00±39.25)μl/ml]、TNF-α[(767.99±33.58)μl/ml vs.(370.14±32.31)μl/ml]和c Tn T[(562.58±33.17)μl/ml vs.(161.56±31.43)μl/ml]水平均降低,差异有统计学意义(P均<0.05)。术后4周,与假手术组比较,模型组和瑞舒伐他汀组大鼠左心室重量、左心室/全心百分比和全心重量均升高,差异有统计学意义(P均<0.05)。与模型组比较,瑞舒伐他汀组干预后左心室/全心百分比[(88.20±3.17)%vs.(73.45±2.31)%]降低,差异有统计学意义(P<0.05),提示瑞舒伐他汀对改善左心室肥厚有一定作用。结论瑞舒伐他汀可改善大鼠急性心肌梗死后炎症反应和左心室肥厚。

Objective To observe the therapeutic effect of rosuvastatin on rats with acute myocardial infarct （AMI）. Methods SD rats （n=60） were randomly divided into sham-operation group only given opening and closing chest treatment and oral normal saline solution once a day for 1 w, rosuvastatin group prepared as AMI model and given rosuvastatin （5 mg/kg） once a day for 1 w, and mode group prepared as AMI model and given normal saline solution i w before operation once a day for 1 w （each n=20）. The number of death rats （died within 1 h after operation） were counted. The expressions of serum interleukin-8 （IL-8）, tumor necrosis factor-α（TNF- α） and cardiac troponin T （cTnT） were detected by using ELISA after operation for 8 h. Results There were 56 survived and 4 died among 60 SD rats. There was 1 died due to respiratory failure and 2 due to arrhythmias in model group, and 1 died due to anesthetic accident in rosuvastatin group. Compared with sham-operation group, the levels of IL-8, TNF-α and eTnT increased in model group and rosuvastatin group （all P〈0.05）. Compared with model group, the levels of IL-8 [（552.91 ± 35.61） μl/mL vs. （264.00 ±39.25） μl/mL], TNF-α [（767.99 ±33.58） μ 1/ mL vs. （370.14± 32.31） μl/mL] and cTnT [（562.58 ± 33.17）μl/mL vs. （161.56 ± 31.43）μ l/roLl decreased in rosuvastatin group after treatment （all P〈0.05）. After operation for 4 w, left ventricular weight （LVW）, percentage of LVW to whole heart weight and whole heart weight increased in model group and rosuvastatin group compared with sham-operation group after treatment （all P〈0.05）. Compared with model group, percentage of LVW to whole heart weight [（88.20 ± 3.17）% vs. （73.45± 2.31）%] decreased in rosuvastatin group （P〈0.05）, which indicated that rosuvastatin had some relieving effect on left ventrieular hypertrophy. Conclusion Rosuvastatin can relieve inflammation and left ventrieular hypertrophy after AMI in rats.