诱骗受体3的二级结构及B细胞表位预测

Prediction of secondary structure and B-cell epitope of decoy receptor 3

1目的预测诱骗受体3(Dcr3)蛋白的二级结构及B细胞表位。2方法通过NCBI蛋白数据库检索到Dcr3蛋白的氨基酸序列,以此为基础,通过SOPMA在线服务器预测其二级结构,利用Lasergene软件预测其亲水性、表面可及性、柔韧性区域及抗原性指数,综合分析,预测其可能的B细胞表位。3结果 Dcr3的二级结构由无规则卷曲(43.00%)、β折叠(10.33%)、α螺旋(36.33%)和β转角(10.33%)构成,其可能的B细胞表位位于56-67(TFVQRPCRRDSP),93-101(VLCGEREEE),56-167(GTFSASSSSSEQ),255-261(LKLRRRL),286-293(PGLERSVR)。4结论此结果为制备Dcr3表位特异性抗体肽段选择提供了依据。

Objective Prediction the secondary structure and B-cell epitope of Dcr3（decoy receptor 3）.Methods The amino acid sequence of Dcr3 protein was retrieved by NCBI protein database.Based on this,the secondary structure was predicted by online service SOPMA;We use software of Lasergene for hydrophilicity,flexibility,accessibility index.Combined the results,we predicted the B cell epitopes of Dcr3.Results The second structure of Dcr3 contained random coil（43.00%）,extended strand（10.33%）,alpha helix（36.33%）,beta turn（10.33%）,and the most possible epitopes of Dcr3 were located in 56-67（TFVQRPCRRDSP）,93-101（VLCGEREEE）,156-167（GTFSASSSSSEQ）,255-261（LKLRRRL）,286-293（PGLERSVR）.Conclusion These results provide a theory basis for developing epitope-specific monoclonal antibodies against Dcr3.