哈维氏弧菌和白斑综合症病毒对墨吉明对虾仔虾的致病性

The Pathogenicity of Vibrio Harveyi and White Spot Syndrome Virus to Penaeus Merguiensis Larva

研究了不同浓度哈维氏弧菌与白斑综合症病毒单独、合并浸泡感染,以及不同温度和盐度条件下单独、合并浸泡感染对墨吉明对虾仔虾死亡情况和WSSV携带量的影响。结果表明:高浓度哈维氏弧菌浸泡感染仔虾,48 h死亡率可达100%,高浓度WSSV浸泡感染仔虾,96 h死亡率可达97%;盐度突变可以降低弧菌与病毒浸泡感染对仔虾的致病性;高温条件下(32℃)弧菌对仔虾的致病性较强,低温条件下(26℃)病毒对仔虾的致病性较强;盐度26、水温26℃条件下,合并感染组死亡率较WSSV单独感染组低;哈维氏弧菌感染可以抑制WSSV在对虾体内的复制。

Penaeus merguiensis larva were immersion infected with Vibrio harveyi and WSSV,both single or combined,at different concentrations under different levels of salinity or different temperature to study its mortality and its load of WSSV. The P. merguiensis larva were found to have 100% mortality after immersion infected with the highest concentration of the V. harveyi at 48h,97% mortality after injected with the highest concentration of the WSSV at 96h. The acute changes of salinity decreased the pathogenicity of V. harveyi and WSSV to shrimp larva. The P. merguiensis larva was more vulnerable to infection of V. harveyi than WSSV under 32 ℃,and the WSSV resulted in more severe damage to shrimp larva at 26 ℃. Combined immersion infection group resulted in less mortality than the WSSV single immersion infection group at the salinity level of 26 and 26 ℃,which indicated that V. harveyi might inhibit the proliferation of the WSSV in the shrimps.