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广藿香中PTS基因不同时间点的表达分析 被引量：8

Expression Analysis of PTS Gene of Pogostemon Cablin at Different Time Points
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摘要 利用荧光定量PCR技术,分析1天中8个时间点广藿香叶片百秋李醇合成酶基因的表达情况。结果表明,6∶00,9∶00,12∶00和15∶00时间点的PTS基因表达量较低,0∶00,3∶00,18∶00和21∶00的表达量均显著高于前4个时间点,其中,时间点3∶00的表达量最高,表明PTS基因的转录从夜间开始积累,黎明前达到最高峰,之后转录水平逐渐下降。 The patchouli alcohol synthase（ PTS） gene was extracted by using real- time PCR technology from the leaves of patchouli（ Pogostemon cablin） at eight time points in a day to study its expression change,The results showed that the expression of PTS gene was low at 6：00 am,9：00 am,12：00 pm and 15：00 pm,but increased much more at 18：00 pm,21：00 pm,0：00 am and 3：00 am. PTS gene transcription started to accumulate at night until it reached a peak before dawn,after which the level of transcription was gradually decreased.
作者 刘璐 吴友根 于靖 张军锋 杨东梅 陈萍
机构地区 海南大学园艺园林学院
出处 《热带生物学报》 2016年第1期89-91,共3页 Journal of Tropical Biology
基金 国家自然科学基金项目(81360618 31360210) 海南省中药现代化专项(ZY201413)
关键词 广藿香 生物钟 百秋李醇合成酶(PTS) Pogostemon cablin circadian clock patchouli alcohol synthase
分类号 S567.2 [农业科学—中草药栽培]
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参考文献7

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二级参考文献19

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热带生物学报
2016年 第1期

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