基于生物效价检测和高效液相色谱法的中药莪术及其炮制品品质评价的研究

Study on quality evaluation of curcuma and its processed products by biopotency determination method and HPLC method

目的建立莪术的生物效价检测指标来评价生莪术、醋煮莪术、醋炙莪术的活血化瘀、消炎功效的差异,并建立高效液相色谱法测定莪术及其炮制品中有效成分姜黄素的含量。方法生物效价测定法将莪术及其炮制品水提取液与牛血纤维蛋白原充分混匀,再加入凝血酶后观察体外凝集反应,并计算莪术活血化瘀效价,高效液相色谱法色谱柱为Waters Cl8(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%甲酸(47∶53),流速为1.0 m L/min,检测波长为420 nm。结果生莪术抗纤维蛋白原的生物效价为2.07U,醋煮莪术为2.58 U,醋炙莪术为1.72 U,而生莪术中主要功效成分姜黄素含量为0.03599 mg/g,醋炙莪术、醋煮莪术中姜黄素含量分别为0.03916 mg/g、0.04167 mg/g。结论以拮抗牛血纤维蛋白原作为生物效价的评价,醋煮莪术的活血化瘀功效高于生莪术和醋炙莪术。

Objective To establish a method for determining anticoagulation potency of Curcuma,Vinegar boiled Curcuma,Vinegar sunburned Curcuma on promoting blood circulation and removing blood stasis,and the establishment of a HPLC method for content determination of effective ingredients of zedoary turmeric curcumin and its processed products. Methods By the full mixture water extracts of these Curcuma and Processed Products and full mixing of fibrinogen,prothrimbin was added into the mixture to from the gel reaction in the nature state and the antagonism titer of Curcuma and Processed Products was calculated. HPLC column for waters C18（ 250 mm × 4. 6 mm,5 μm）,the mobile phase is acetonitrile 0. 2% formic acid（ 47∶53）. The flow rate was 1. 0 m L / min and the detection wavelength was 420 nm. Results The biological potency of antifibrin Curcuma for2. 07 U,Vinegar boiled Curcuma was 2. 58 U and Vinegar sunburned Curcuma was 1. 72 U. And zedoary main efficacy components of the content of curcumin 0. 03599 mg / g,vinegar Rhizoma Zedoariae and vinegar boiled the content of curcumin in Rhizoma Curcumae respectively 0. 03916 mg / g and0. 04167 mg / g. Conclusion Evaluation of the antagonistic activity of fibrinogen as a biological activity,the Vinegar boiled Curcuma＇s effection of activating blood circulation is higher than the Vinegar sunburned Curcuma and the Curcuma.