从肝治心组方对大鼠缺血心肌血管新生及HGF蛋白表达的影响

Study on the Effects of Conggan Zhixin Prescription(从肝治心组方) on Angiogenesis of Ischemic Myocardium and HGF in Rats

目的:观察从肝治心组方(CGZXP)对冠心病心肌缺血大鼠局部缺血心肌HGF蛋白表达,以及对缺血心肌血管新生的影响。方法:将SD大鼠随机的分成假手术组、模型对照组、从肝治心组方组和麝香保心丸组。采用冠状动脉结扎法诱导急性心肌梗死模型,造模24 h后给药,1次/d。于造模14 d后处死大鼠,取出心脏,肉眼观察心肌情况,应用免疫组织化学法进行缺血心肌HGF蛋白表达的检测。结果:模型对照组、从肝治心组方组及麝香保心丸组HGF蛋白表达均显著高于假手术组,差异均有统计学意义(P<0.05或P<0.01);从肝治心组方组及麝香保心丸组HGF蛋白表达均显著高于模型对照组,差异均有统计学意义(P<0.05);从肝治心组方组HGF蛋白表达高于麝香保心丸组,但差异无统计学意义(P>0.05)。结论:从肝治心组方可促进冠心病心肌缺血大鼠HGF表达,促进血管新生,具有保护缺血心肌的作用。

Objective： To observe the effects of Conggan Zhixin Prescription （从肝治心组方 CGZXP） on myocardial ischemic of HGF protein expression and ischemic myocardial angiogenesis in rats. Methods： According to completely random method, SD rats were divided into 4 groups： sham-operated group, model group, Shexiang Baoxin pill （从肝治心组方 SXBXP） group and CGZXP group. Acute myocardial infarction animal models were induced by ligating the coronary artery, after model rats were made for 24 hours, giving them medicine 1 times per day. Rats were killed 14 days after modeling, the heart was removed, and cardiac conditions were observed by naked-eyes. Then use immunohistochemistry to determinate and detect angiogenesis and HGF protein expression of ischemic myocardial. Results： The expression of HGF of model group, CGZXP group and SXBXP group was higher than the sham-operated group （P〈0.05 or P〈0.01）. And the expression of HGF of CGZXP group and SXBXP group was higher than the model group （P〈0.05）. The expression of HGF of CGZXP group was higher than SXBXP group, but the difference was not statistically significant （P〉0.05）. Conclusion： CGZXP can effectively increase the expression of HGF and promote neovascularization in ischemic myocardium, and protect ischemic myocardium.