不同途径裸鼠大肠癌肝转移模型的比较

Models of colorectal cancer liver metastasis in nude mice

目的建立并比较四种不同途径的裸鼠大肠癌肝转移模型,从而根据不同需要选择较为理想的裸鼠模型。方法 BALB/c（nu/nu）裸鼠共80只随机分为4组（每组20只）,分别经脾脏背膜下、肝脏背膜下、盲肠浆膜下、门静脉注射5×10 6个HCT116细胞建立大肠癌肝转移模型,观察并记录各组小鼠生存状态,小鼠死亡后进行剖腹探查观察腹腔内肿瘤生长、肝脏转移情况,对肿瘤组织进行常规病理组织学观察。结果脾脏被膜下注射组、肝被膜下注射组、盲肠浆膜下注射组注射位置均成功成瘤;肝转移率：脾被膜下注射组为94.7%,盲肠浆膜下注射组为44.4%,门静脉注射组为66.7%;各模型的原发肿瘤及转移瘤均为典型的低分化腺癌。肝被膜下注射组肿瘤进展迅速,门静脉注射组围手术期死亡率高,盲肠浆膜下注射组生存时间较长。结论四种模型具有各自的优势和缺点,应根据实验需要来选择恰当的动物模型。

Objective To compare four different models of colorectal cancer liver metastasis in nude mice. Methods Eighty BALB / c（ nu / nu） mice were randomly divided into spleen subcapsular injection group（ 20 mice）,liver subcapsular injection group（ 20）,cecum subserosal injection group（ 20） and intraportal injection group（ 20）. 5 × 10~6HCT116 cells were injected into mice respectively. Living status of mice were documented,primary tumors and metastases were carefully observed after mice died,as well as tumor histopathological examination. Results Primary tumors were injected successfully in all of 4 groups. Of the mice,94. 7% developed with liver metastasis in the spleen subcapsular injection group,44. 4% in the liver subcapsular injection group,and 66. 7% in the cecum subserosal injection group. Typical poorly-differentiated adenocarcinoma was observed in both primary tumors and metastases by histopathological examination. Rapidly involved tumors were observed in the liver subcapsular injection group,and a high perioperative mortality rate was observed in the intraportal injection group. But a longer survival rate was observed in the cecum subserosal injection group. Conclusion Four animal models have their own advantages and disadvantages,and should be chosen according to experiment purpose.