疟原虫遗传减毒子孢子诱导抗肺癌免疫的初步研究

Antitumor effect of malaria genetically attenuated sporozoites infection in a murine lewis lung cancer model

目的初步探讨疟原虫遗传减毒子孢子能否诱导抗肺癌免疫作用,为肺癌疫苗的研究提供新的思路。方法实验分为实验组与对照组,实验组尾静脉注射遗传减毒子孢子进行免疫C57BL/6J小鼠,对照组注射磷酸缓冲盐溶液(PBS);14d后用Lewis肺癌细胞(LLC)皮下接种,待肿瘤长出后用游标卡尺测量肿瘤大小;肿瘤组织免疫组化对比肿瘤增殖、凋亡、血管生成情况。结果两组小鼠的肿瘤生长有差异,疟原虫遗传减毒子孢子能抑制肿瘤的增殖、血管生成、促进凋亡。结论遗传减毒子孢子可能是一种新的治疗策略或能成为一种有效的载体介导肺癌的免疫治疗。

Objective To learn whether plasmodium genetic attenuated sporozoites（GAS）can induce immunity against lung cancer,in order to provide new ideas for the study of lung cancer vaccine.Methods Ther study was divided into two groups respectively,experimental group received intravenous injection of genetically attenuated sporozoites to immunize C57BL/6Jmice and control group injection of phosphate buffer solution（PBS）;after 14 days,we subcutaneously inoculated lewis lung cancer（LLC）cells,calipers was used to measure tumor size.Immunohistochemical staining was detected tumor proliferation,apoptosis,and angiogenesis.Results There was statistically significant in tumor size.Immunohistochemical staining revealed that attenuated sporozoites infection inhibited LLC eslls proliferation,angiogenesis,apoptosis.Conclusion The malaria attenuated sporozoites may provide a novel strategy or therapeutic vaccine vector for anti-lung cancer immune-based therapy.