枸杞多糖对小鼠宫颈癌细胞增殖的影响

Effect of lycium barbarum polysaccharide on proliferation of mouse cervical cancer cells

目的探讨枸杞多糖(LBP)对小鼠宫颈癌细胞增殖的影响及其机制。方法 MTT法检测不同浓度LBP作用48h后对体外培养的宫颈癌U14细胞的抑制率。ELISA法检测LBP 1000μg/ml作用48h后U14细胞分泌IL-12及IL-10的水平。构建20只C57BL/6J小鼠U14细胞皮下成瘤模型,随机均分为两组,实验组每日灌胃LBP 10mg/kg,对照组予等量生理盐水。给药4周后ELISA法检测两组小鼠血清IL-12及IL-10的水平;计算小鼠瘤质量、体积、胸腺指数和脾脏指数;免疫组化法检测瘤块中Ki-67的表达。结果 LBP呈剂量依赖性地抑制U14细胞的生长。LBP 1000μg/ml作用后,U14细胞IL-12分泌增加,IL-10分泌减少(P<0.01)。与对照组比较,实验组小鼠血清IL-12水平、胸腺和脾脏指数升高,血清IL-10水平、瘤质量、体积及Ki-67表达量降低(P<0.01)。结论 LBP能够通过上调IL-12及下调IL-10的水平达到抗小鼠宫颈癌细胞增殖的作用。

Objective To investigate the effect and underlying mechanism of lycium barbarum polysaccharide（LBP）on the proliferation of mouse cervical cancer cells.Methods MTT was used to detect the inhibitory rates of cervical cancer cell U14 in vitro,which was treated with different concentrations of LBP for 48 hours.ELISA was used to detect the levels of IL-12 and IL-10 secreted by U14 incubated with LBP 1000μg/ml for 48 hours.Twenty C57BL/6J mice with subcutaneous xenografts of U14 were equally randomized into two groups.Group A was daily fed with LBP10mg/kg and group B was daily fed with same volume of normal saline as the control.After four weeks,ELISA was used to detect serum levels of IL-12 and IL-10 in mice.The tumors,thymus and spleen were separated from the mice and tumor mass and volume,thymus index and spleen index were calculated.The Ki-67 staining was used to detect the proliferation of U14.Results LBP could inhibit the growth of U14 in a dose-dependant manner in vitro.The secretion of IL-12 was increased,while that of IL-10 was decreased from U14 after incubated with LBP 1000μg/ml for 48 hours.Compared with group B,serum level of IL-12,thymus index and spleen index were increased,while serum level of IL-10,tumor mass and volume,and expression level of Ki-67 were decreased in group A（P〈0.01）.Conclusion LBP is able to inhibit the proliferation of mouse cervical cancer cell U14 through upregulating the level of IL-12 and downregulating that of IL-10.