丹参-人参组分配伍对肺癌A549细胞侵袭迁移及ERK1/2磷酸化水平的影响

Effects of Component Formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome on Cell Migration,Invasionand and Phosphorylation of ERK1/2 in Lung Cancer A549 Cells

目的研究丹参-人参组分配伍对肺癌A549细胞侵袭迁移以及ERK1/2磷酸化水平的影响。方法采用细胞划痕实验和高内涵细胞成像系统分析丹参-人参组分配伍对肺癌A549细胞迁移的影响;应用实时细胞传感电阻仪Transwell实验动态检测丹参-人参组分配伍对肺癌A549细胞侵袭的影响;通过纳米级超微量蛋白质分析系统检测丹参-人参组分配伍对肺癌A549细胞ERK1/2磷酸化水平的影响。结果丹参-人参组分配伍可显著增加划痕空白区域的距离(P<0.01),且呈一定的时间依赖性;显著降低细胞迁移的面积(P<0.01),且呈一定的剂量依赖性;对A549细胞侵袭有抑制作用(P<0.01),且呈一定的时间依赖性;能够显著降低肺癌A549细胞ERK1/2磷酸化水平(P<0.01)。结论丹参-人参组分配伍能显著降低肺癌A549细胞迁移侵袭的能力,其作用机制可能与降低肺癌A549细胞ERK1/2磷酸化水平有关。

OBJECTIVE To investigate the effects of component formula of Salviae miltiorrhizae radix et rhizome and Ginseng panax et rhizome on cell migration,invasion and phosphorylation of ERK1/2in lung cancer A549 cells.METHODS The scratch wound healing assay and high content screening were used to analyze the effects of component formula of Salviae Miltiorrhizae Radix Et Rhizoma and Ginseng Radix Et Rhizome on A549 cell migration.The Real-time cell analysis was adapted to detect the effects of component formula on A549 cell invasion.The phosphorylation of ERK1/2in lung cancer A549 cells was measured by nanoscale ultramicro protein analysis system.RESULTS Component formula treatment significantly increased the distance of scratch blank area in time-dependent manner（P〈0.01）,compared with control group;decreased A549 cells migration area（P〈0.01）in a dose-dependent pattern;significantly inhibited A549 cells invasion（P〈0.01）in time-dependent manner;and decreased the phosphorylation of ERK1/2（P〈0.01）in A549 cells.CONCLUSION The component formula of Salviae Miltiorrhizae Radix Et Rhizome and Ginseng Panax Et Rhizome can inhibit A549 cells migration and invasion,which may be related to the decreased phosphorylation level of ERK1/2.