玉竹百合蛤蜊汤煎煮工艺及抗疲劳作用的研究

Study on Decoction Process and Antifatigue Effect of Polygonatum Lilium Meretrix Decoction

目的研究经典食疗方玉竹百合蛤蜊汤的最佳煎煮方法及其抗疲劳的作用,为今后以该方为基础的抗疲劳保健品的开发提供理论依据。方法为优选玉竹百合蛤蜊汤的煎煮工艺,以浸膏得率,总多糖、牛磺酸的含量为考察指标,以加水量、提取时间、提取次数为考察因素,采用正交试验优选其煎煮方法;并按照2003版《保健食品检验与评价技术规范实施手册》的要求,设正常组、模型组、阳性组及玉竹百合蛤蜊汤低、中、高3个剂量组,经口给予样品,采用小鼠游泳疲劳模型,对比各组小鼠负质量游泳时间、不负质量游泳后血清乳酸(LA)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、丙二醛(MDA)指标的差异,观察玉竹百合蛤蜊汤对小鼠的抗疲劳能力影响。结果玉竹百合蛤蜊汤最佳煎煮工艺为加10倍量水,提取3次,每次60min,在最佳煎煮工艺下,总多糖含量为(79.48±7.82)mg/g,牛磺酸含量为(1.95±0.02)mg/g,干浸膏得率为(26.82±0.53)%。高、中剂量组的玉竹百合蛤蜊汤能显著延长小鼠负质量游泳时间(P<0.05);与模型组相比,3个剂量组的玉竹百合蛤蜊汤能显著降低小鼠血清乳酸(P<0.01)及丙二醛(P<0.01)的含量,且呈剂量依赖性,并能显著降低肌酸激酶(P<0.01)、乳酸脱氢酶(P<0.01)的活力,并升高超氧化物歧化酶的活力。结论本实验确定了玉竹百合蛤蜊汤的最佳煎煮工艺,并证明了该食疗方具有较好的抗疲劳功效,为该方作为保健食品进一步开发提供依据。

OBJECTIVE To determine the best water-coction extract technology of Polygonatum Lilium Meretrix Decoction（PLMD）and investigate the anti-fatigue effect of PLMD in mice.METHODS The preperation condition of PLMD was optimized by orthogonal design with extractive yield,amount of polysaccharides and taurine as index.At the same time,mice were randomly divided into six groups：normal group,model group,positive group and administrated groups.In administrated groups,mice were given different doses of PLMD.Swimming model was established after orally administrated daily for 15 days,loaded-swimming time and serum indicators were conducted to explore PLMD＇s antifatigue effect.RESULTS The best decoction technique was adding 10-fold of water,decocting three times 60 minutes each time,in which the polysaccharides and taurine content was（79.48±7.82）,（1.95±0.02）mg/g crude drug,respectively,extractive yield was（26.82±0.53）%.The medium and high doses group of PLMD could obviously prolong the loaded-swimming time,significantly decreased LA,MDA,LDH,CK levels,and the enzyme activity of SOD was ehhanced in dose groups than in model group.CONCLUSIONThe PLMD＇s best decoction process was identified and its antifatigue effect was also confirmed.This result provided the basis for the further development of PLMD.