黄芩不同器官及愈伤组织中3种黄酮类成分和总黄酮的含量比较

Comparison of Three Kinds of Flavonoids and Total Flavonoids Contents in Different Organs of Scutellaria Baicalensis Geogi and Its Callus

目的比较黄芩植株不同器官（根、茎、叶、花）及黄芩药材（子芩、枯芩、绿芩）和黄芩愈伤组织中野黄芩苷、黄芩苷、汉黄芩苷和总黄酮的含量。方法采用SYMMETRY C18（4.6mm×250mm,5μm）柱,以乙腈（A）-0.22%乙酸水溶液（B）为流动相,柱温35℃,波长335nm,检测3种黄酮类成分的含量;采用紫外分光光度法,以芦丁为对照品,采用硝酸铝显色后于504nm处测定其总黄酮含量。结果野黄芩苷、黄芩苷、汉黄芩苷在质量浓度为6.0～192.0、30.0～960.0、12.0～384.0μg/mL范围内与峰面积呈良好线性关系（R2≤0.998 9）,平均加样回收率为97.8%～103.1%,RSD≤4.0%。总黄酮在质量浓度为40.00～240.00μg/mL范围内线性关系良好（R2≥0.999 8）,平均加样回收率为98.8%,RSD为1.0%。结论黄芩地上部分（花、茎、叶）的野黄芩苷含量高于地下部分（根）,以花中的含量为最高,达到0.53%;地下部分的黄芩苷、汉黄芩苷含量高于地上部分,愈伤组织中黄芩苷达到0.60%。总黄酮含量以愈伤组织中含量最高,达到30.42%。为利用黄芩废弃部位花提取野黄芩苷和利用愈伤组织生产黄酮提供了依据。

OBJECTIVE To compare the contents of three kinds of flavonoids（scutellarin,baicalin and wogonoside）and total flavonoids in different organs of Scutellaria baicalensis Georgi（root,stem,leave anf flower）,medicinal herbs of Scutellaria（tender Scutellaria,old Scutellaria and green Scutellaria）and its callus.METHODS SYMMETRY C18（4.6mm×250mm,5μm）column was used with the solvent system of acetonitrile（A）-0.22% acetic acid water（B）and the UV detection wavelength of 335 nm,at the temperature of 35℃,to detect the contents of three kinds of flavonoids.The contents of total flavonoids were determined with the control sample of rutin,the indicators of aluminum nitrate and the UV detection wavelength of 504 nm.RESULTS The linear ranges of scutellarin,baicalin and wogonoside were 6.0～192.0,30.0～960.0,12.0～384.0μg/mL（R2≤0.998 9）,respectively.The average recoveries were 97.8%～103.1%,RSD≤4.0%.The linear ranges of total flavonoids was 40.0～240.0mg/mL（R2≥0.999 8）,and the average recoveries was 98.8%（RSD=1.0%）.CONCLUSION The content of scutellarin in aboveground Scutellaria baicalensis Georgi（flower,stems and leaves）was higher than underground part（root）,and the content in flowers is highest,reaching the point of 0.53%.Baicalin and wogonoside content in underground part of are higher than aboveground,and the content in callus baicalin reaches 0.60%.In callus,the content of total flavonoid is highest（30.42%）.Those results may provide a basis for scutellarin extraction fromScutellariaflower and the production of total flavonoids fromScutellariacallus.