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Cre-loxp系统的构建及在牙釉质发育中的应用

The Construction of Cre-loxp System and Its Application in Enamel Development
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摘要 Cre-loxp系统是近年来广泛应用在各领域的重组酶系统。该系统由组织特异性重组酶cre和loxp位点组成。可以通过对特定的DNA序列进行准确的切割及连接,达到在基因水平上对生物体进行定向遗传改造的目的。牙釉质的发育离不开各种转录因子的作用,利用该系统对各转录因子的缺失或突变的研究,探讨特定时间、特定部位目的基因的异常改变对牙釉质发育的影响。 Cre-loxp system is recombinant enzyme systems,widely used in various fields in recent years.The system consists of a tissue-specific recombinase cre and loxp site components.Through specific DNA sequences accurate cutting and connection,at the genetic level to achieve the purpose of orienting genetically engineered organisms.Enamel development is inseparable from the role of various transcription factors,utilization of the system for each missing or mutated transcription factors,investigate the effect of abnormal changes in a particular time parts of the gene of enamel development.
作者 褚青 高玉光
机构地区 滨州医学院附属医院
出处 《中外医学研究》 2016年第11期160-162,共3页 CHINESE AND FOREIGN MEDICAL RESEARCH
关键词 Cre-loxp系统 LOXP位点 重组酶cre 转录因子 Cre-loxp system Loxp site Recombinase cre Transcription factors
分类号 R781 [医药卫生—口腔医学]
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参考文献18

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二级参考文献40

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中外医学研究
2016年 第11期

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