酒续断对骨质疏松型大鼠OPG/RANK/RANKL轴系统的调控研究

Study on regulation of wine-processed Dipsacus on OPG/RANK/RANKL axis system in osteoporosis rats

目的比较生、酒续断灌胃给药对骨质疏松大鼠骨保护素(OPG)/细胞核因子-κB受体活化因子(RANK)/RANK配体(RANKL)轴的调控差异。方法将30只购于上海杰思捷实验动物有限公司的SPF级SD大鼠通过去势法造成骨质疏松大鼠模型,随机分为正常组、假手术组、模型组、雌二醇组、生续断组和酒续断组,每组5只,灌胃给药12周后,麻醉主动脉取血,采用全自动生化分析仪对给药12周后大鼠血清中钙(Ca)、磷(P)、碱性磷酸酶、骨钙素4项基本指标进行分析,同时使用酶联免疫吸附试验法检测血清OPG、RANK和RANKL 3项骨代谢指标水平,并进行组间对比分析。结果与模型组比较,雌二醇组、生续断组和酒续断组大鼠的碱性磷酸酶、骨钙素和RANKL水平均显著降低,OPG和RANK水平均显著升高,酒续断组大鼠的Ca水平显著降低,P水平升高,差异均有统计学意义(P<0.05);与雌二醇组比较,生续断组大鼠的碱性磷酸酶和骨钙素水平显著升高,酒续断组大鼠的Ca水平显著降低,P和骨钙素水平显著升高,生续断组和酒续断组大鼠的OPG和RANK水平均显著降低,RANKL水平均显著升高,差异均有统计学意义(P<0.05);与生续断组比较,酒续断组大鼠的Ca、碱性磷酸酶、骨钙素及RANKL水平均显著降低,OPG和RANK水平均显著升高,差异均有统计学意义(P<0.05)。结论续断对骨质疏松大鼠的治疗作用与其调控OPG/RANK/RANKL轴有关,酒续断的调控能力强于生续断。

Objective To compare the regulation difference of gavage administration of crude and wine-processed Dipsacus on OPG/RANK/RANKL axis in osteoporosis rat. Methods Thirty SPF grade SD rats purchased from the Shanghai Jiesijie Laboratory Animal Co.,Ltd. were established the osteoporotic model by the castration method and randomly divided into the nor-mal group,sham operation group,model group,estradiol group,crude Dipsacus group and wine-processed Dipsacus group,5 cases in each group. After 12 weeks of gavage administration,the rats were anesthetized and aortic blood was collected. The basic biochemical indicators of serum Ca,P,alkaline phosphatase and osteocalcin were analyzed by adopting the automatic biochemical analyzer. Meanwhile,the 3 bone metabolic indicators of serum OPG,RANK and RANKL levels were determined by using the ELISA method. Results Compared with the model group,the levels of alkaline phosphatase,osteocalcin and RANKL in the estradiol group,crude Dipsacus group and wine-processed Dipsacus group were significantly reduced,while the levels of OPG and RANK were significantly increased,the Ca level in the wine-processed Dipsacus group was significantly reduced and the P level was increased,the differences were statistically significant（P〈0.05）;compared with the estradiol group,the levels of alkaline phosphatase and osteocalcin in the crude Dipsacus group were significantly increased,whereas the Ca level in the wine-processed Dipsacus group was significantly reduced and the levels of P and osteocalcin were significantly increased,the levels of OPG and RANK in the crude and wine-processed Dipsacus groups were significantly reduced, while the RANKL level was significantly elevated,the differences were statistically significant（P〈0.05）;compared with the crude Dipsacus group,the levels of Ca,alkaline phosphatase,osteocalcin and RANKL levels in the wine-processed Dipsacus group were significantly reduced,while the levels of OPG and RANK were significantly increased with statistical differences（P〈0.05）. Conclusion The curative effect of Dipsacus in treating osteoporosis rat is related with its regulating the OPG/RANK/RANKL axis. The regulation ability of wine-processed Dipsacus is stronger than that of crude Dipsacus.