摘要
聚乙烯吡咯烷酮(PVP)是一种重要的非渗透性低温保护剂。本文使用低温显微镜,研究了不同浓度(10%、20%和30%)PVP水溶液在不同降温速率(1℃/min、10 ℃/min、50℃/min和100℃/min)的结晶形态以及初始结晶温度。研究结果表明,在较低浓度条件下结晶颗粒较大,容易出现冰沟壑;在较高浓度条件下,冰晶颗粒相对较小,未见明显的冰沟壑产生;此外,初始结晶温度随着降温速率的增加而降低。在适当的高浓度条件下提升降温速率可以有效减少结晶量,有利于低温保存。
Polyvinylpyrrolidone（PVP） is an important impermeable cryoprotectant.Cryomicroscope was used to study the initial crystallization temperatures and the crystalline forms of Polyvinylpyrrolidone aqueous solution with different concentrations（10%,20% and 30%） at different cooling rates（1℃/min,10℃/min,50℃/min and 100℃/min）.The results show that,the sizes of crystals are relatively bigger and the ice gap tends to take shape when PVP is at lower concentration; the sizes of crystals are relatively smaller when PVP is at higher concentration,and no obvious ice gap occurs; moreover,the initial crystallization temperature decreases with the increase of cooling rate.Crystals can be effectively reduced by accelerating the cooling rate when PVP is at higher concentration,which is beneficial to cryopreservation.
出处
《制冷技术》
2016年第1期31-34,共4页
Chinese Journal of Refrigeration Technology
基金
国家自然科学基金资助(No.81300554)
关键词
聚乙烯吡咯烷酮
结晶
降温速率
低温保存
Polyvinylpyrrolidone
Crystallization
Cooling rate
Cryopreservation
