雷公藤多苷对咪喹莫特诱导银屑病样小鼠Wnt/Frizzled信号传导通路的影响

Effect of tripterygium glycosides on Wnt/Frizzled signaling pathway in imiquimod-induced psoriasis-like mice

目的：研究雷公藤多苷对咪喹莫特诱导银屑病样小鼠Wnt/Frizzled信号传导通路的影响。方法：选择BALB/c雌性小鼠作为研究对象,随机分为对照组、模型组以及干预组,模型组以及干预组建立咪喹莫特诱导银屑病样小鼠的模型,干预组在建模后给予雷公藤多苷灌胃。取银屑病皮损组织,测定Wnt/Frizzled信号分子及下游相关分子的含量。结果：模型组小鼠皮损组织中Wnt5a、Frizzled2、Frizzled3、Frizzled5、Frizzled6、NFAT、COX-2、VEGF、MIF、IFN-γ、IL-6、IL-17、IL-21、IL-23、JAK1、STAT3、Rsa、Raf、MEK、ERK1、EKR2的含量明显高于对照组,cGMP、PKG的含量明显低于对照组,Frizzled4的含量与对照组无差异;干预组小鼠皮损组织中Wnt5a、Frizzled2、Frizzled3、Frizzled5、Frizzled6、NFAT、COX-2、VEGF、MIF、IFN-γ、IL-6、IL-17、IL-21、IL-23、JAK1、STAT3、Rsa、Raf、MEK、ERK1、EKR2的含量明显低于模型组,cGMP、PKG的含量明显高于模型组,Frizzled4的含量与模型组无差异。结论：雷公藤多苷对咪喹莫特诱导银屑病样小鼠皮损组织中Wnt5a-Frizzled2/Frizzled3/Frizzled5/Frizzled6所介导的信号通路具有抑制效应。

Objective：To study the effects of tripterygium glycosides on Wnt/Frizzled signaling pathway in imiquimodinduced psoriasis-like mice.Methods：BALB/c female mice were selected as research objects and randomly divided into control group,model group and intervention group.Model group and intervention group were established the models of imiquimod-induced psoriasis-like mice,and intervention group received intragastric administration of tripterygium glycosides after establishment of models.Psoriasis lesion tissue was collected to detect the contents of Wnt/Frizzled signal molecules and downstream related molecules.Results：Wnt5a,Frizzled2,Frizzled3,Frizzled5,Frizzled6,NFAT,COX-2,VEGF,MIF,IFN-γ,IL-6,IL-17,IL-21,IL-23,JAK1,STAT3,Rsa,Raf,MEK,ERK1 and EKR2contents in skin lesion tissue of model group were significantly higher than those of control group,cGMP and PKG contents were significantly lower than those of control group,and Frizzled4 content was not different from that of control group;Wnt5a,Frizzled2,Frizzled3,Frizzled5,Frizzled6,NFAT,COX-2,VEGF,MIF,IFN-γ,IL-6,IL-17,IL-21,IL-23,JAK1,STAT3,Rsa,Raf,MEK,ERK1 and EKR2contents in skin lesion tissue of intervention group were significantly lower than those of model group,cGMP and PKG contents were significantly higher than those of model group,and Frizzled4 content was not different from that of model group.Conclusion：Tripterygium glycosides have inhibitory effects on the signaling pathway mediated by Wnt5a-Frizzled2/Frizzled3/Frizzled5/Frizzled6 in skin lesions of imiquimod-induced psoriasis-like mice.