糖尿病视网膜病变发病的内质网应激机制及羟苯磺酸钙的治疗作用研究

Role of endoplasmic reticulum stress in the pathogenesis of diabetic retinopathy and effects of calcium dobesilate on the disease

目的：本研究对糖尿病视网膜病变发病的内质网应激机制及羟苯磺酸钙的治疗作用进行研究,为临床治疗提供参考。方法：对本院诊治的糖尿病视网膜病变患者120例进行研究,分析羟苯磺酸钙治疗前后患者血清中内质网应激蛋白及白细胞介素蛋白表达水平的变化。以医院同期健康体检者55例为对照组。结果：研究发现糖尿病视网膜病变组患者血清中内质网应激蛋白PERK、CHOP和IRE及白细胞介素蛋白IL-1、IL-2、IL-6和IL-10表达明显升高,血清中MDA水平明显升高而SOD、CAT和GSHpx水平明显降低,且与对照组相比,差异具有统计学意义（P〈0.01）;经羟苯磺酸钙治疗后上述因子得到明显的恢复（P〈0.01）。结论：糖尿病视网膜病变与内质网应激密切相关,且羟苯磺酸钙可能是通过抑制内质网应激发挥改善糖尿病视网膜病变的作用。

Objective：To explore the role of endoplasmic reticulum stress in the pathogenesis of diabetic retinopathy and effects of calcium dobesilate on the dieases in order to provide reference for clinical treatment.Methods：A total of 120 cases of diabetic retinopathy patients treated in our hospital were studied.The serum endoplasmic reticulum stress protein and interleukin protein expression levels were analyzed before and after Calcium dobesilate treatment.Another 55 cases of healthy volunteers undergoing physical examination in our hospital during the same period were taken as control group.Results：Expression levels of serum endoplasmic reticulum stress proteins including PERK,CHOP and IRE as well as interleukin proteins IL1,IL2,IL6 and IL10 were significantly increased,serum MDA level significantly increased while SOD,CAT and GSHpx levels significantly decreased in diabetic retinopathy patients showing significant statistical differences（P〈0.01）.After Calcium dobesilate treatment,above factors were all significantly restored（P〈0.01）.Conclusion：Diabetic retinopathy is closely related to endoplasmic reticulum stress and Calcium dobesilate treatment may improve diabetic retinopathy by inhibiting endoplasmic reticulum stress.