高效液相色谱法同时测定麦冬中3个黄酮成分的含量

Simultaneous Determination of Three Flavone Constituents in Ophiopogonis Radix by HPLC Method

目的建立测定麦冬药材中3个具有代表性的黄酮成分,甲基麦冬高黄酮A、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B的高效液相色谱法。方法采用HPLC-DAD,色谱柱为Comatex C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(58∶42),流速1.0 m L·min-1,柱温30℃,检测波长296 nm,进样量20μL。结果甲基麦冬高黄酮A、麦冬甲基黄烷酮A和甲基麦冬二氢高异黄酮B 3种成分的线性方程分别为Y=493 321ρ+31 262(r=0.999 9)、Y=605 744ρ+40 941(r=0.999 9)、Y=586 672ρ+39 657(r=0.999 9);平均回收率分别为100.59%(RSD=1.51%)、99.27%(RSD=1.28%)、100.04%(RSD=1.33%)。结论建立的麦冬3种黄酮含量测定方法准确灵敏、重复性好,可应用于麦冬药材质量评价中。

OBJECTIVE To establish an HPLC method for simultaneous determination of methylophiopogonone A,methylophiopogonanone A,and methylophiopogonanone B. METHODS Comatex C18column（ 4. 6 mm × 250 mm,5 μm） was used for the HPLC analysis. The mobile phase consisted of acetonitrile and water（ 58∶ 42） and was eluted at the flow rate of 1 mL·min^- 1. The column temperature was maitained at 30 ℃. The detection wavelength was set at 280 nm. The injection volume was 15. 0 μL. RESULTS The linear regression equations of methylophiopogonone A, methylophiopogonanone A and methylophiopogonanone B were Y = 493 321ρ ＋ 31 262（ r = 0. 999 9）,Y = 605 744ρ ＋ 40 941（ r = 0. 999 9）,and Y = 586 672ρ ＋ 39 657（ r = 0. 999 9）,respectively.The average recovery rates of the three flavones respectively were 100. 59%（ RSD = 1. 51%）,99. 27%（ RSD = 1. 28%）,and100. 04%（ RSD = 1. 33%）. CONCLUSION The established method for simultaneous determination of flavone constituents in Ophiopogonis Radix is accurate and sensitive,with good repeatability. It can be applied to the quality evaluation of Ophiopogonis Radix.