Wnt5b在葛根素促进成骨分化中的作用

The role of Wnt5b in the promotion of osteogenic differentiation by puerarin

目的:探索对骨髓间充质干细胞(BMMSCs)具有最佳促成骨能力的葛根素浓度以及与Wnt信号通路之间的相关性。方法:取3周龄的SD大鼠,分离培养BMMSCs,传至第3代后接种,改用含不同浓度葛根素的培养基。继续培养2~4周,测定碱性磷酸酶与骨钙素的含量,评价葛根素的体外诱导成骨能力。用Elisa试剂盒测定Wnt5b的含量,评估葛根素的促成骨作用与Wnt信号通路的关系。采用SPSS16.0软件包对数据进行统计学分析。结果:不同浓度组的细胞ALP、OCN和Wnt5b含量增加,10^(-5) mol/L组显著高于对照组,高于其他浓度组,但差异无显著性。结论 :葛根素能有效促进BMMSCs向成骨方向分化,以10^(-5) mol/L浓度效果最佳,其作用机制可能与Wnt信号通路有关。

PURPOSE： To investigate the capability of puerarin on osteogenic induction in bone marrow-mesenchymal stem cells（BMMSCs）, and to explore the mechanism of osteoinductivity of puerarin in vitro. METHODS： After segregating and cultivation, bone marrow stromal cells from 3-weeks SD rats were induced by osteogenesis medium containing different concentrations of puerarin in induction groups; while in non-induction group, BMMSCs were still cultured with DMEM medium. Following continued culture for 2-4 weeks, alkaline phosphatase activity, osteocalcin and Wnt5 b were measured. The capability of puerarin on osteogenic induction of BMMSCs and the relationship between this effect and Wnt signaling pathway were investigated. SPSS 16.0 software package was used for statistical analysis.RESULTS： After induction with puerarin, the level of alkaline phosphatase activity, osteocalcin and Wnt5 b increased.10^（-5）mol/L group showed the best effect. CONCLUSIONS： Puerarin can induce BMMSCs differentiation into osteoblasts.The mechanism may be related to Wnt signaling pathway.