减毒结核分枝杆菌H37Ra感染小鼠模型的实验研究

Study on the mouse model infected by Mycobacterium tuberculosis H37Ra

目的:研究减毒结核分枝杆菌(M.tb)H37Ra感染小鼠模型及其病理损伤的机制。方法:将清洁级Balb/c小鼠分为对照组和M.tb模型组,各3只。将H37Ra按照每只小鼠1×106CFU的剂量,经尾静脉注射感染小鼠,分别于感染7、14和21 d后,无菌操作取小鼠肺和脾脏组织,测量肺和脾脏的脏器系数,匀浆后行活菌培养;肺和脾脏组织冷冻切片,行HE染色观察病理损伤情况。结果:小鼠感染M.tb 14和21 d后,脾脏脏器系数均较感染7 d后显著升高(P<0.01和P<0.05)。感染7 d后,肺和脾脏组织匀浆均可检出活菌,脾脏组织H37Ra活菌量明显高于肺脏,并随感染时间逐渐增加;感染14 d后,肺组织病理观察可见明显感染性病变损伤。结论:M.tb H37Ra经尾静脉注射感染小鼠模型可造成部分结核病样病理性损伤。

Objective： To Study on the pathological lesion in Balb/c mice infected with H37 Ra. Methods： The mice were divided into control group and model group infected with Mycobacterium tuberculosis. Balb / c mice was injected via the tail vein with attenuated Mycobacterium tuberculosis H37Ra（ 1 × 106 CFU / mouse）. After infection,living bacteria,organ weight index of lung or spleen,and histopathological lesion were detected after 7,14,and 21 days of infection. Results： Compared with the group of control,the organ coefficient of spleen increased statistically after 14 days or 21 days of infection（ P 〈0. 01 and P〈 0. 05）; The number of living bacteria in spleen tissue of infected mice increased with the days of infection and was higher in spleen tissue than that in lung tissue.Tuberculosis-like lesions were observed in spleen and lung tissue of infected mice model. Conclusions： Attenuated Mycobacterium tuberculosis H37 Ra could induced tuberculosis-like lesions in Balb / c mouse via the tail vein injection.