香青兰总黄酮对血管紧张素Ⅱ诱导血管平滑肌细胞增殖与迁移的影响

Inhibitory effects of dracocephalum total flavones against proliferation and migration of vascular smooth muscle cells induced by AngⅡ

目的:研究香青兰总黄酮对血管紧张素Ⅱ(AngⅡ)诱导的大鼠主动脉血管平滑肌细胞(VSMC)增殖与迁移的作用。方法:采用贴壁法培养大鼠胸主动脉平滑肌细胞,以AngⅡ为诱导剂,建立VSMC细胞增殖的模型,分别应用质量浓度为10^(-7) mol·L^(-1) AngⅡ以及AngⅡ+不同浓度香青兰总黄酮组(25,50,100 mg·L^(-1))作用24 h,并设空白对照组进行比较。采用MTT法检测细胞的增殖;transwell法检测细胞的迁移;免疫组化法检测细胞内增殖细胞核抗原(PCNA)的表达水平。结果:与对照组比较,AngⅡ组能显著刺激大鼠VSMC的增殖和迁移,香青兰总黄酮不同剂量组联合AngⅡ可在一定程度上抑制AngⅡ诱导的VSMC增殖,迁移以及细胞内PCNA的表达,且呈现一定的剂量依赖关系趋势。结论:香青兰总黄酮具有抑制AngⅡ诱导VSMC增殖与迁移的作用。

OBJECTIVE To explore the possible mechanism of pharmacological activity of tilianin by observing the effects of Dracocephalum total flavoes on proliferation and migration of rat vascular smooth muscle cells（VSMCs）induced by AngⅡ.METHODS VSMC were primary cultured by tissue sticking method.Cell proliferation model was established by stimulation with AngⅡ,Cell proliferation model as established by stimulation with 10-7 mol·L-1 AngⅡ.After AngⅡstimulation,cells were treated with three dose of Dracocephalum total flavoes（25,50,100 mg·L-1）of 24 hours,and a control group;MTT assay was used to evaluate the proliferation of cells.Transwell assay was used to evaluate the migration of cells.The expression of intracellular proliferating cell nuclear antigen（PCNA）were measured by immunohistochemistry.RESULTS Compared with control group,three dose groups of Dracocephalum total flavoes could inhibit,in varying degrees,the proliferation,migration and expression of intracellular PCNA of VSMCs induced by AngⅡ,in a dose-dependent manner.CONCLUSION The Dracocephalum total flavoes could inhibit the proliferation and migration of VSMC induced by AngⅡ.