大鼠眼玻璃体腔注射人脐带间充质干细胞后致眼内感染的实验研究

Experimental study of endophthalmitis resulted from rat intravitreal injection of human umbilical cord mesenchymal stem cells

目的比较不同方式玻璃体腔注射人脐带间充质干细胞(hUC-MSCs)后眼内感染率,为寻找减少眼内感染的注射方式。方法采用酶消化法进行人脐带间充质干细胞提取,流式细胞仪检测其表面标志CD73、CD105、Anti-HLA-ABC、CD34、CD45、Anti-HLA-DR;将60只SD大鼠120只眼随机分为上方玻璃体腔注射组和下方玻璃体腔注射组,各60只眼;对感染眼内炎的大鼠抽取玻璃体,进行细菌培养检测。结果 120只大鼠眼中5眼出现感染性眼内炎,总感染率为4.2%,其中4只眼为下方注射组,1只眼为上方注射组,两组的感染率分别为6.7%及1.7%,两组比较差异有统计学意义(P<0.05);感染病原菌为金黄色葡萄球菌2株、表皮葡萄球菌2株、链球菌属1株。结论选择上方玻璃体腔注射是比较安全的方式,可能会减少眼内感染的发病率。

OBJECTIVE To compare incidences of endophthalmitis with different methods of intravitreal injection of human umbilical cord mesenchymal stem cells and to explore a more secure injection way to reduce endophthalmitis.METHODS Enzyme digestion method was used to extract hUC-MSCs,flow cytometry was used to detect surface marks including CD73,CD105,anti-HLA ABC,CD34,CD45,anti-HLA-DR.Totally 60 healthy SD rats（120eyes）were randomly divided into superior injection group and inferior injection group.Vitreous humors were extracted from rats infected with endophthalmitis and were used for bacterial culture detection.RESULTS A total of five eyes developed infectious endophthalmitis and total infection rate was 4.2%.Among them,four eyes were injected inferiorly and one eye was injected superiorly.The infection rates respectively were 6.7% and 1.7%.Between two groups,there was statistically difference（P〈0.05）.Bacterial culture results showed that there were 2cases of Staphylococcus aureus,2cases of Staphylococcus epidermis,and 1case of Streptococcus.CONCLUSION Superior intravitreal injections is a relatively safe way and may reduce the rate of endophthalmitis.