蛹虫草多肽对大鼠急性酒精性肝损伤的保护作用

Protective effect of Cordyceps militaris polypeptide against acute alcoholic liver injury in rats

目的探讨蛹虫草多肽对急性酒精性肝损伤大鼠肝脏的保护作用及机理。方法将60只Wistar大鼠随机分为5组,即空白对照组、模型对照组、低剂量蛹虫草多肽组、中剂量蛹虫草多肽组和高剂量蛹虫草多肽组。除空白对照组外,每组均给予10ml/kg、56°白酒灌胃,1次/d;空白对照组给予相同剂量蒸馏水每日灌胃。模型对照组和不同剂量给药组每日给予白酒灌胃后1 h,分别给予蒸馏水及蛹虫草多肽溶液(6 ml/kg)灌胃,4周后眼眶取血。检测各组大鼠血清中ALT和AST水平、肝脏内超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平;并在光镜下观察肝脏病理结构变化。计量资料多组间比较采用方差分析,进一步两两比较采用SNK-q检验。结果与模型组比较,不同剂量给药组大鼠血清中ALT和AST水平及肝脏中MDA水平均明显下降(P值均<0.05),肝脏中SOD活性均显著提高(P值均<0.05),且不同剂量给药组间比较差异均有统计学意义(P值均<0.05);给药组肝脏病理切片镜下可见酒精所致肝细胞脂肪变性及坏死等损伤减轻。结论蛹虫草多肽对酒精造成的急性肝损伤具有保护作用,作用机理可能与其抗氧化作用相关。

Objective To investigate the protective effect of Cordyceps militaris polypeptide against acute alcoholic liver injury in rats and related mechanism. Methods A total of 60 Wistar rats were randomly divided into blank control group,model group,and low-,medium-,and high- dose Cordyceps militaris polypeptide groups. All rats except those in the blank control group were given 10 ml / kg 56° liquor by gavage once a day; the rats in the blank control group were given distilled water of the same dose by gavage once a day. At 1 hour after gavage with liquor,the rats in the model group and low-,medium-,and high- dose Cordyceps militaris polypeptide groups were given distilled water or Cordyceps militaris polypeptide solution（ 6 ml / kg） by gavage. Blood samples were collected from the orbit 4 weeks later.The serum levels of alanine aminotransferase（ ALT） and aspartate aminotransferase（ AST） and the activity of superoxide dismutase（ SOD）and level of malondialdehyde（ MDA） in the liver were measured for each group,and the pathological changes in the liver were observed under a light microscope. Analysis of variance was applied for comparison between multiple groups,and the SNK- q test was applied for comparison between any two groups. Results Compared with the model group,the low-,medium-,and high- dose Cordyceps militaris polypeptide groups showed significant reductions in the serum levels of ALT and AST and the level of MDA in the liver（ all P〈0. 05）,as well as a significant increase in the activity of SOD in the liver（ all P〈0. 05）,while these indices showed significant differences between the low-,medium-,and high- dose Cordyceps militaris polypeptide groups（ all P〈0. 05）. The liver pathological sections from the low-,medium-,and high- dose Cordyceps militaris polypeptide groups showed alleviated hepatocyte fatty degeneration and necrosis induced by alcohol under a light microscope. Conclusion Cordyceps militaris polypeptide has a protective effect against acute liver injury induced by alcohol,and its mechanism of action may be related to its antioxidant effect.