山西省血液中心参加中国国际输血感染预防和控制室间质评的结果分析

Analysis of the results of external quality assessment of China international transfusion infection control(CITIC) of blood center of Shannxi province

目的分析2013年我实验室参加中国国际输血感染预防和控制(CITIC)室间质评的结果,验证实验室检测输血传染病标志物的能力,提高实验室检测水平。方法将实验室CITIC室间质评的结果与目标结果进行比较,验证检测方法的符合性、灵敏度和特异性。结果 2013年实验室参加CITIC室间质评3次,检测CITIC室间质评标本75个,其中第1次室间质评的结果与目标结果不一致的标本有2个,总符合率为98%。结论 HBs Ag ELISA(生物梅里埃)方法检测标本M乙肝表面抗原结果与目标结果不一致的原因为标本在发放过程中快递出现故障,标本长时间存放于室温导致结果不准确;抗-HCV ELISA(索林)方法检测标本C丙肝抗体结果与目标结果不一致的原因为试剂批间差异造成的假反应性,其他结果均与目标结果符合。2013年我实验室参加CITIC室间质评的结果符合预期。

Objective To analyze the results of CITIC external quality assessment scheme in which the blood screening laboratory had participated in 2013,by which we can validate the competence of the laboratory and improve its detection ability. Methods To investigate the consistency of the results from CITIC and our laboratory,and to compare the sensitivity and specificity between these results. Results Our laboratory participated in CITIC external quality assessment scheme for three times in 2013,and 75 specimens supplied by the institution of CITIC were tested in our laboratory. The results of 2 in 75 specimens tested in our laboratory were not consistent with real results from CITIC,in which the total coincidence rate was 98%. Conclusion The test results of 73 in 75 specimens in our laboratory were consistent with the real results from CITIC. Delivery delay maybe the reason for the inconsistent results in the test of specimen M with HBs Ag ELISA kit（Bio Merieux）. The specimen M was keep in room temperature too long time to get an accurate result. The test result of specimen C in our laboratory was inconsistent with the real result from CITIC because of the difference among anti-HCV ELISA kit（Dia Sorin）batches。Hence,the result that our laboratory participated in CITIC external quality assessment scheme in 2013 is in accordance with the expected one.