HPLC法测定浙江慈溪水稻田栽培的西红花中西红花苷Ⅰ、Ⅱ含量的研究

Content Determination of Crocin Ⅰ and Crocin Ⅱ in Crocus sativus L.Cultivated from Rice Paddy in Cixi,Zhejiang Province by HPLC

为了检测栽培于浙江慈溪水稻田中的西红花所含的西红花苷Ⅰ和西红花苷Ⅱ的含量,采用高效液相色谱法,色谱条件为Intertsil ODS-SP C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(60∶40),检测波长440nm,流速1.0 m L/min,进样量为20μL,柱温为室温。结果表明:西红花花柱中西红花苷I的平均含量为8.85%,西红花苷Ⅱ的平均含量为3.72%;雄蕊中西红花苷I的平均含量为0.094%,西红花苷Ⅱ的平均含量为0.005%;而花瓣中西红花苷I和西红花苷Ⅱ的含量极少,未能检出。因此,栽培于浙江慈溪水稻田中的西红花花柱中西红花苷I和西红花苷Ⅱ两者含量之和为12.57%,符合2010版《中国药典》的质量标准,可以作为药用。

To determine the content of crocin Ⅰ and crocin Ⅱ in Crocus sativus L. cultivated from rice paddy in Cixi,Zhejiang Province. In this paper,the active ingredients of different parts of saffron were detected by high performance liquid chromatography（ HPLC）. And Inertsil ODS- SP C18 chromatographic column（ 150 mm × 4. 6 mm,5 μm） was used. The mobile phase was methanol and water（ 60∶ 40）. The detection wavelength was 440 nm,the flow rate was 1. 0 m L / min,the injection volume was 20 μL and the column temperature was room temperature. The results showed that the average content of crocin I was 8.85% and the average content of crocin Ⅱ was 3. 72% in style of saffron. The average content of crocin Ⅰ was 0. 094% and the average content of crocin Ⅱ was 0. 005% in stamen of saffron. While the content of crocin I and crocin Ⅱ in petal of saffron was few,it did not be detected. The both content of crocin I and crocin Ⅱ was 12. 57% in style of saffron,which met the quality of the 2010 edition of China Pharmacopoeia standard. So the saffron cultivated from rice paddy in Cixi,Zhejiang province can be used as medicine.