β-葡聚糖对巨噬细胞摄取氧化低密度脂蛋白的影响

Effects of β-glucan on the uptake of oxLDL in macrophages

目的：探讨β-葡聚糖对巨噬细胞源性泡沫细胞形成的影响及相关机制。方法：以RAW264.7巨噬细胞为细胞模型,MTT法检测β-葡聚糖对巨噬细胞细胞活性的影响;油红O染色观察β-葡聚糖对巨噬细胞摄取氧化低密度脂蛋白（oxygenized low density lipoprotein,ox LDL）的影响;Western blot检测β-葡聚糖对巨噬细胞SR-A、CD36、c-fos、c-Jun蛋白表达的影响。采用SPSS 10.0统计软件分析数据,并采用双尾t检验进行统计学意义评估。结果：2.5~10μg/m Lβ-葡聚糖对巨噬细胞细胞活性无影响;5μg/m Lβ-葡聚糖显著抑制巨噬细胞对ox LDL的摄取,从而减少巨噬细胞源性泡沫细胞的形成;与对照组比较,2.5~10μg/m L的β-葡聚糖剂量依耐性降低巨噬细胞SR-A蛋白及细胞核蛋白c-Jun的表达（P〈0.05）,但对CD36蛋白及细胞核蛋白c-fos表达无影响（P〉0.05）;与5μg/m Lβ-葡聚糖处理组比较,c-Jun抑制剂SP600125进一步增强β-葡聚糖抑制细胞SR-A蛋白表达的作用（P〈0.05）。结论：β-葡聚糖可抑制巨噬细胞源性泡沫细胞的形成,且与抑制c-Jun/SR-A通路相关。

Objective： To explore the effects and the mechanisms of β-glucan on the formation of macrop-hage-derived foam cells. Methods： The effects of β-glucan on cell viability was examined by MTT. Oil-red O staining was used to observe the effects of β-glucan on the uptake of ox LDL, and the effects of β-glucan on SRA、CD36、c-fos、c-Jun protein expression in macrophages were investigated using Western blot. Data were analyzed using SPSS 10.0 statistical software and two-tailed t test was used for statistical significance assessment.Results： β-glucan ranged from 2.5 ~ 10 μg /m L did not affect cell viability, and at 5 μg/m L inhibited the formation of macrophage-derived foam cells via attenuating ox LDL uptake by macrophages. Compared with control group, β-glucan（2.5 ~ 10 μg /m L） treatment dose-dependently decreased the expression of SR-A and nuclear c-Jun without affecting that of CD36 and nuclear c-fos in macrophages. Compared with β-glucan at 5 μg/m L group, SP600125（c-Jun inhibitor） significantly augmented the inhibitory effects of β-glucan on SR-A expression（P〈 0.05）. Conclusion： β-glucan could inhibit the formation of macrophage-derived foam cells, which are possibly related to the inactivation of c-Jun/SR-A pathway.