双醋瑞因有关物质的HPLC法测定

Determination of the Related Substances in Diacerein by HPLC

建立了高效液相色谱法测定双醋瑞因的9个有关物质。采用Phenomenex Prodigy 100A ODS3色谱柱,以乙腈∶25 mmol/L磷酸二氢钾溶液（用磷酸调至pH 2.2）（42∶58）为流动相,检测波长254 nm。双醋瑞因及其有关物质（除七乙酰芦荟苷）在0.2~3.0mg/ml范围内线性关系良好,七乙酰芦荟苷在0.6~3.0mg/ml浓度范围内线性关系良好。有关物质的平均回收率96.8%~99.9%,RSD为1.7%~3.5%。

An HPLC method was established for the determination of the nine related substances in diacere. A Phenomenex prodigy 100 A ODS3 column was used with the mobile phase of acetonitrile∶25 mmol/L potassium dihydrogen phosphate solution（adjusted to p H 2.2 with phosphoric acid）（42∶58）, at the detection wavelength of 254 nm. It was linear for diacerein and its related substances（except heptaacetyl barbaloin） in the range of 0.2 — 3.0 μg/ml. As for heptaacetyl barbaloin, the linear range was 0.6 — 3.0 μg/ml. The average recoveries of the related substances were 96.8%— 99.9%, with RSDs of 1.7%— 3.5%.