广西汉族人群Toll样受体91486T/C基因多态性与ANCA相关性小血管炎的相关性

An association study of Toll- like receptor gene 1486T / C polymorphism with primary antineutrophil cytoplasmic antibodies associated small vasculitis

目的探讨广西汉族人群TLR9 1486T/C基因多态性与原发抗中性粒细胞胞浆抗体(ANCA)相关性小血管炎(AAV)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测116例AAV患者(AAV组)和209例性别、年龄相匹配的健康成人(对照组)TLR9 1486T/C位点多态性,并检测其血清生化指标。结果 AAV组与对照组TLR9 1486T/C位点基因型和等位基因分布比较差异无统计学意义(P>0.05)。AAV患者三组基因型间尿素氮、肌酐、Ig M水平比较差异有统计学意义(P<0.05)。病理资料分析显示AAV患者三组基因型小血管炎活动指数及慢性指数差异无统计学意义(P>0.05)。结论广西汉族人群中,TLR9 1486T/C基因多态性可能与尿素氮、肌酐、Ig M升高具有相关性,可能与AAV的遗传易感性不相关。

Objective To investigate the association of TLR9 gene 1486T/C polymorphism with primary antineutrophil cytoplasmic antibodies（ ANCA） associated small vasculitis（ AAV） in a Guangxi population with Han ethnicity.Methods TLR9 1486 T / C polymorphism was analyzed by polymerase chain reaction- restricted fragments length polymorphism（ PCR- RFLP） in 116 cases of AAV patients and 209 cases of age and gender matched normal control. Serum biochemical parameters were tested. Results No significant difference was found in either genotype distributions or allele frequencies between the patients and the controls（ P〉0. 05）. Significant difference were observed in blood urea nitrogen,serum creatinine and Ig M levels among the three genotypes of patients（ P〈0. 05）. In AAV patients,there was no statistical significant difference in activity index and chronic index among the three genotypes（ P〉0. 05）. Conclusion In AAV patients,TLR9 1486 T / C gene polymorphism may be associated with elevated blood urea nitrogen,serum creatinine and Ig M levels. CC,TT and CT genotypes were detected in TLR9 1486 T / C in patients with AAV in Guangxi,without significant correlation with genetic susceptibility to primary AAV.