肿瘤相关巨噬细胞与卵巢癌组织之间的关系及预后的影响

The correlation of tumor-associated macrophages with ovarian cancer tissue and its prognosis

目的本研究旨在进一步研究阐明肿瘤相关巨噬细胞特异性标志物CD68相对表达量与卵巢癌发生发展及预后的关系。方法纳入我院2012年1月~2015年1月卵巢癌手术切除患者的病例样本共计48例,对这48例卵巢癌病例采用逆转录实时定量聚合酶链反应RT-PCR的方法对肿瘤相关巨噬细胞特异性标志物CD68的相对表达量进行检测。结果肿瘤相关巨噬细胞特异性标志物CD68相对表达量与患者的年龄、分化程度、肿瘤侵袭深度及瘤组织大小、是否转移等并不存在相关性（P〉0.05）;而淋巴受累方面,淋巴受累组的肿瘤相关巨噬细胞相对表达量明显下调（P〈0.05）,无淋巴受累组的相对表达量与正常组相比差异无统计学意义（P〉0.05）,肿瘤相关巨噬细胞相对表达量明显下调与淋巴结受累转移的风险有关;生存分析方面,肿瘤相关巨噬细胞相对表达量明显下调的患者中生存时间较高表达的患者明显增长。结论肿瘤相关巨噬细胞相对表达量水平与卵巢癌的淋巴结转移及预后有着相关性。

Objective To further clarify the correlation of the relative expression amount of tumor-associated macrophage specific marker CD68 with the occurrence, development, and prognosis of ovarian cancer. Methods 48 patients who received surgical resection of ovarian cancer from January 2012 to January 2015 were selected. With the real-time quantitative reverse transcriptase-polymerase chain reaction（RT-PCR） technique, the relative expression amounts of tumor-associated macrophage specific marker CD68 in all patients were detected. Results There was no correlation of the relative expression amounts of tumor-associated macrophage specific marker CD68 with the age,tumor differentiation, tumor invasion depth, tumor tissue size, and tumor metastasis of patients（P〈0.05）.In the lymphatic involvement group, the relative expression amounts of tumor-associated macrophage specific marker CD68 obviously declined（P〈0.05）, while the relative expression amount differences between the normal group and the group without lymphatic involvement was not statistically significant（P〉0.05）.The significant reduction in the relative expression amount of tumor-associated macrophage was associated with the metastasis risk of lymph node involvement. In terms of survival analysis, there was an obvious increase in the number of the patients with higher survival time among the patients who had a reduction in the relative expression amount of tumor-associated macrophage. Conclusion The relative expression level of tumor associated macrophage has a correlation with the lymph node metastasis in ovarian cancer and its prognosis.