杏鲍菇遗传多样性的SRAP和ITS分析

Genetic Diversity Analysis of Pleurotus Eryngii by SRAP and ITS Markers

研究来自中国不同地区和日本的27株杏鲍菇栽培菌株的遗传多样性,以期为杏鲍菇的鉴定和选育提供分子依据。利用SRAP和ITS标记联合使用的方法,通过聚类分析对供试杏鲍菇菌株进行研究。筛选出9对SRAP引物共扩增出151条条带,其中具有多态性条带130条,平均多态性比例为83.3%,多态性信息含量（PIC）变幅在0.27~0.42,平均为0.36。通过ITS序列对供试菌株进行亲缘关系分析,与SRAP分析的结果一致,均表明地域来源相近的部分菌株聚在一起,亲缘关系较近,而地域相隔较远的部分菌株也聚为一类,其亲缘关系也很近。2种标记均显示供试杏鲍菇栽培菌株的遗传多样性较为丰富,其遗传相似性与地理分布存在一定的联系。SRAP和ITS联合使用能够得到更好的效果,从而为杏鲍菇遗传多样性研究提供更为有力的参考。

Genetic diversity of 27 Pleurotus eryngii cultivating strains from different areas in China and Japan were analyzed to provide a molecular evidence for the identification and seed selection of Pleurotus eryngii.The tested isolates were studied using clustering analysis, combined with SRAP and ITS markers. 9 pairs ofprimers on 27 strains were selected, and 151 fragments were amplified, polymorphic bands were 130, theaverage polymorphic rate was 83.3%. The PIC（polymorphism information content） value of these markersvaried from 0.27 to 0.42, averaging 0.36. Kinship of the tested strains was determined by analyzing their ITSsequences, and the result which was consistent with SRAP analysis, showed that part of the strains from thenearby areas were clustered with each other, their kinship was close, while some strains with large gaps in thegeographical distribution were also clustered in the same phylogenetic tree branch, their genetic relationshipmay also be relatively close. Both of the two markers showed that the genetic diversity of the tested strains wasrelatively abundant, and there was a certain link between genetic similarity and geographical distribution. Themethod which combined SRAP with ITS could get better results, and it provided a powerful reference for the study on the genetic diversity of Pleurotus eryngii.