化湿行瘀清热方剂治疗口腔扁平苔藓前后血清中差异蛋白的初步研究

Differentially expressed serum proteins in oral lichen planus patients before and after treated with Huashi Xingyu Qingre Decoction

目的口腔扁平苔藓(oral lichen planus,OLP)是发生于口腔黏膜的常见病,目前尚无确切疗法。文中主要探讨化湿行瘀清热方剂对OLP的血清中差异蛋白变化情况。方法随机选取2013年10月至2015年5月间于河北医科大学第四医院口腔科确诊经化湿行瘀清热方剂治疗的30例OLP患者。抽取治疗前后空腹静脉血,离心获取血清并提取总蛋白。采用双向荧光差异凝胶电泳和液相色谱-质谱联用技术筛选、鉴定与OLP相关的差异蛋白,并通过蛋白免疫印迹法验证。结果化湿行瘀清热方剂治疗OLP前、后的血清中存在结合珠蛋白、人抗凝血酶Ⅲ、补体成分C1和维生素D结合蛋白4种差异蛋白。治疗后,患者血清中的结合珠蛋白表达水平(159.704±24.228)较治疗前(103.086±27.536)升高,人抗凝血酶Ⅲ表达水平(98.00±28.04)较治疗前(150.00±54.04)降低,差异均有统计学意义(P<0.05)。结论化湿行瘀清热方剂治疗OLP前、后血清中的结合珠蛋白、人抗凝血酶Ⅲ、补体成分C1和维生素D结合蛋白的表达存在差异,对OLP的发生、发展存在一定的影响作用。

Objective Oral lichen planus（ OLP） is a common complaint in the oral mucosa,for which there is no definite therapy hitherto. This article aimed to investigate the possible effect of Huashi Xingyu Qingre Decoction（ HXQD） on OLP. Methods This study included 30 randomly selected cases of OLP treated with HXQD. Fasting venous blood and total serum protein were obtained before and after medication for screening and identification of OLP-related differential proteins by two-dimensional fluorescence difference gel electrophoresis（ 2D DIGE） and liquid chromatography-mass spectrometry（ LC-MS）,followed by Western blot validation.Results Haptoglobin,antithrombin Ⅲ,C1 complement,and vitamin D binding protein were differentially expressed in the serum of the OLP patients before and after treated with HXQD. Compared with the baseline,the expression of haptoglobin was significantly increased（ 103. 086 ± 27. 536 vs 159. 704 ± 24. 228,P 〈0. 05） while that of antithrombin Ⅲ remarkably decreased after treatment（ 150. 00 ± 54. 04 vs 98. 00 ± 28. 04,P 〈0. 05）. Conclusion Haptoglobin,antithrombin Ⅲ,C1 complement,and vitamin D binding protein are differentially expressed in the serum of OLP patients before and after HXQD medication,which may be associated with the development and progression of OLP.