2014年部分地区猪流行性腹泻病毒流行病学调查

Epidemiological survey of porcine epidemic diarrhea virus in 2014

为了解猪流行性腹泻病毒(PEDV)的流行情况,本研究利用RT-PCR方法对2014年来自全国11个省市42个猪场的165份病料样品进行了检测。结果显示,在检测的42个猪场中,共有36个猪场显示PEDV阳性,猪场阳性率为85.71%。其中单独感染PEDV的猪场有25个,占总数的59.52%;混合感染PEDV和猪传染性胃肠炎病毒(TGEV)的猪场有2个,占总数的4.76%,PEDV和猪轮状病毒(Po RV)混合感染猪场有7个,占总数的16.67%;3种病毒均混合感染的猪场有2个,占总数的4.76%。结果表明PEDV在我国的猪腹泻病病原中仍占主导地位。对2014年分离的21株PEDV ORF3基因进行测序分析,并与Gen Bank中登录的12株国内外PEDV参考株相关序列进行遗传变异分析。这33个PEDV株的ORF3基因系统发育树显示,目前我国的PEDV现地病毒株分布在Ⅰ群中的Ⅰ-Ⅰ亚群和Ⅰ-Ⅲ亚群。

To investigate the prevalence of porcine epidemic diarrhea virus（PEDV） in China, a total of 165 clinical samples were collected from 42 pig farms in 11 provinces in 2014 and detected for PEDV by the RT-PCR. The results showed that the positive rate of PEDV in the pig farms was 85.71%（36/42）, of which the positive rates were 59.52%（25/42） for PEDV single infection, 4.76%（2/42） for PEDV and porcine transmissible gastroenteritis（TGEV） co-infection, 16.67%（7/42） for PEDV and porcine rotavirus（Po RV） co-infection, and 4.76%（2/42） for PEDV, TGEV and Po RV co-infection. The results indicated that PEDV still was the main pathogen causing the pig diarrhea in China. In addition, the ORF3 genes of the 21 PEDVs isolated from the positive samples were sequenced and compared with the 12 reference strains in the Gen Bank. The phylogenetic analysis demonstrated that the current epidemic PEDVs were classified PEDV group I and further divided into subgroup I-I and I-III.