青蒿组织培养再生体系的建立

The Establishment of Regeneration System of Tissue Culture of Artemisia annua L.

为了建立和优化青蒿的组织培养再生体系,获得快速繁殖技术,以青蒿叶片和叶柄为外植体,研究了不同激素配比对其离体培养和大量快速繁殖的影响。结果表明:以青蒿叶片为外植体,其愈伤组织诱导培养基为MS+2.0mg/L 6-BA+0.5mg/L 2,4-D,诱导率为78.0%;分化培养基为MS+1.0mg/L 6-BA+0.05mg/L NAA+3%蔗糖+0.7%琼脂粉,分化率达95.3%。分化苗在1/2MS+1.0mg/L NAA培养基上快速生根,且根系发达,粗壮,生根率达98.9%。以青蒿叶柄为外殖体,叶柄可在培养基MS+2.0mg/L 6-BA+0.5mg/L 2,4-D上一次成苗,在1/2MS+1.0mg/L NAA培养基上生根良好。该体系有效的缩短了培养周期,具有重要的研究价值。

In order to investigate the callus induction and the regeneration of the plant of Artemisia annua L.,to obtain rapid reproduction technology,the leaves and petioles of Artemisiaa nnua L.were taken as explants,and the MS culture was chosen as the basic culture medium to be used in different combinations with hormone.The results showed that using leaves of Artemisia annua L.as explants,the callus induced medium was MS＋2.0mg/L 6-BA＋0.5mg/L 2,4-D,the induction rate was 78.0%;the differentiation medium was MS＋1.0mg/L 6-BA＋0.05mg/L NAA＋3%sucrose＋0.7%agar powder,the differentiation rate was 95.3%.The differentiated seedling rooted well in the culture medium 1/2MS＋1.0mg/L NAA,the rooting rate was 98.9%.Petioles of Artemisia annua L.could be a seedling by one step in medium MS＋2.0mg/L 6-BA＋0.5mg/L 2,4-D.The system could effectively shorten the training period,and was worthy of study.