不同抗性苹果砧木叶片接种轮纹病菌后防御酶活性的变化

Change of Defensive Enzyme Activity of Leaves Inoculated by Botryosphaeria berengriana.f.sp.piricolaof Different Resistance Apple Rootstock

以高感砧木3-1-29-4和高抗砧木1-1-10-8为试材,对其叶片接种轮纹病菌(Botryosphaeria berengriana.f.sp.piricola),测定了接种后防御酶活性的变化。结果表明:轮纹病菌侵染后,叶片的过氧化物酶(POD)和过氧化氢酶(CAT)的活性均表现为先升后降的趋势,且抗性砧木1-1-10-8的活性高于高感砧木3-1-29-4。叶片的超氧化物歧化酶(SOD)和苯丙氨酸解氨酶(PAL)的活性在接种后持续升高。接种后,抗病砧木1-1-10-8的多酚氧化酶(PPO)活性先升高后降低,高感砧木3-1-29-4的PPO活性持续升高。

Taking a high sensitive rootstock 3-1-29-4and high resistant rootstock 1-1-10-8as materials,and the leaves were inoculated with Botryosphaeria berengriana.f.sp.piricola.After inoculation,the changes of defensive enzymes were studied.The results showed that the activities of peroxidase（POD）and catalase（CAT）in two apple rootstock leaves both rised at first and then declined.And the enzyme activities of resistant rootstock 1-1-10-8were higher than that of sensitive rootstock 3-1-29-4.The activities of superoxide dismutase（SOD）and phenylalanine ammonia-lyase（PAL）steadily increased.The activity of polyphenol oxidase（PPO）of resistant rootstock 1-1-10-8increased at first and then decreased,and the activity of PPO of resistant rootstock 3-1-29-4steadily increased.