利用芯片式高场非对称波形离子迁移谱技术快速检测苯丙氨酸

Rapid Determination of Phenylalanine by Micro-chip based Field Asymmetric Waveform Ion Mobility Spectrometry

采用金属扩散管-芯片式高场非对称波形离子迁移谱（FAIMS）技术对苯丙氨酸进行了快速检测,设定测试压强为250 kP a,金属扩散管温度为190℃,在优化的最佳分析条件下,即：载气流速为2000 mL/min,分离电压为152.8 V时,在正模式下获得了苯丙氨酸的离子特征谱图和补偿电压特征值＂0.62 V。另外,利用FAIMS对不同浓度的苯丙氨酸样品气进行了检测,确定了FAIMS检测的定量线性范围为6~20 mg/L和检出限为5.9 mg/L。本实验为FAIMS应用于苯丙氨酸的快速检测提供了重要参考。

Field asymmetric ion mobility spectrometry（ FAIMS） technology was used for the rapid analysis of phenylalanine（ PHE） using a metal diffusion tube-microchip FAIMS system. The test pressure was set at 250 k Pa,and the temperature of the gas generator for the diffusion tube was at 190℃. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL / min,a dispersion voltage of 152. 8 V and a compensation voltage value of ＂0. 62 V for phenylalanine in positive mode.Phenylalanine gas samples with different concentrations were analyzed by FAIMS. The results showed that the ion intensity was linearly correlated to phenylalanine concentration in the range from 6 mg / L to 20 mg / L,and the limit of detection for phenylalanine was 5. 9 mg / L. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple and sensitive,and can be used for rapid detection of phenylalanine.