非图案化法制备柔性连续葡萄糖监测传感器

Fabrication of Flexible Continuous Glucose Monitoring Sensor by Non-Patterning Method

以聚多巴胺作为前驱体,在聚酰亚胺薄膜表面化学沉积一层牢固致密的金层,以该镀金薄膜两面的金层作为基体电极,在其中一面镀铂黑作为参比-对电极,在另一面依次电沉积铂黑层、电泳形成Nafion/碳纳米管网络层、电吸引形成葡萄糖氧化酶层构成工作电极,并整体涂覆聚氨酯外膜,制备了一种柔性葡萄糖传感器。考察了此传感器对葡萄糖的检测性能,以及各层表面形态结构对传感器性能的影响。传感器在0.3 V的工作电位下对葡萄糖的线性响应范围是2.0~32.0 mmol/L,响应灵敏度为25μA·(mmol/L)^(-1)·cm^(-2),检出限为0.05 mmol/L(S/N=3),且传感器有良好的长期稳定性和抗干扰性,可用于皮下连续血糖监测。

A tight gold layer was electroless plated on polyimide film coated with polydopamine. A polyurethane coated flexible glucose sensor was developed based on this two side gold layer structure,wherein the platinum black plating side was used as reference-counter electrode and another side that was successively modified by platinum black layer,Nafion / carbon nanotube（ CNT） layer and glucose oxidase（ Gox） layer as working electrode. The morphology of each layer and its influence on glucose sensing performance were investigated. The glucose biosensor showed a linear range from 2. 0 to 32. 0 mmol / L and the sensitivity was25 μA·（ mmol / L）^（-1）·cm^（-2）at low potential of 0. 3 V,with a detection limit of 0. 05 mmol / L（ S / N = 3）.The biosensor could avoid the commonly coexisted interference and exhibited high stability in the continuous subcutaneous glucose monitoring.