一株溶藻真菌的分离鉴定及其作用机理研究

Isolation, Identification and Algicidal Mechanism of an Algae-lysing Fungus

从巢湖水体中分离得到了一株具有明显溶藻效果的溶藻真菌,命名为P1,对该菌株溶解铜绿微囊藻(Microcystis aeruginosa)的效果、溶藻方式进行了研究,利用16S r DNA序列分析对其进行了鉴定。当加入培养4天的P1菌液6天后,铜绿微囊藻的去除率可达到84.7%。该菌株溶藻无需菌体与藻细胞直接接触,是通过分泌某种非蛋白质类物质溶藻。16S r DNA序列分析表明,P1菌株与一株霉菌的16S r DNA核苷酸序列的同源性均在100%,归属于霉菌属。

One strain of algae-lysing fungus which had obvious algae-lysing effect was isolated from the Lake Chao, and the Algae-Lysing fungus was named P1, Its algicidal mechanism and effects on Microcystis aeruginosa were studied. When added the fungus liquid of P1 to the Microcystis aeruginosa, the removal of Microcystis aeruginosa was 84.7 % after 6 days. The algae-lysing effects on the Microcystis aeruginosa of fungus P1 did not require physical contact between cells, but depended on the production of extracellular chemicals which are not proteins. The sequence analysis of the 16 S r DNA of strain P1 showed that it shares 100 % sequence homology with one strain of Penicillium. According that, P1 belongs to phylogenetic fungus of Penicillium classification.