摘要
精原干细胞为体内天然干细胞 ,如能体外培养建系或建立其长期培养系统 ,将会极大地促进精原干细胞移植、转基因等技术的实际应用 ,也会给体外研究细胞的发育分化提供很好的材料。影响精原干细胞体外存活、增殖的因素很多 ,该文主要综述了基本培养基的选择、血清和添加剂、激素和维生素的应用及 SCF、L IF、b FGF等生长因子的作用等 ,以期促进精原干细胞的研究。
Spermatogonial stem cells are natural stem cells in vivo. If these cells are immortalized or can be cultured for a long time in vitro, application of spermatogonial stem cells transplantation and gene transfer will be greatly improved, and also a prominent cell line for development and differentiation research will be provided. The survival and proliferation of spermatogonial stem cells in vitro involve many agents. In the present review, selections of primary media, serum, additives, hormones, vitamins and growth factors such as Stem Cell Factor (SCF), Leukocyte Inhibition Factor (LIF) and basic fibroblast growth factor (bFGF) were discussed in order to facilitate internal researches of spermatogonial stem cells.
出处
《动物医学进展》
CSCD
2002年第5期28-30,共3页
Progress In Veterinary Medicine
基金
军队卫生科研基金资助项目 (0 1MA2 0 4)
吉林省科委自然基金资助项目 (2 0 0 10 5 5 5 )
