正交试验优选生姜总黄酮的提取工艺

Optimum Extraction of Total Flavonoid from Ginger Established by Orthogonal Test

以生姜为原料,用乙醇作提取剂,采用单因素和正交实验,运用微波辅助处理浸提法,探讨生姜中提取黄酮类化合物的最佳提取工艺条件:微波功率、微波加热时间、浸提温度、浸提时间等。实验结果表明,微波处理与乙醇提取相结合的方法的最适工艺参数是微波功率200 W、微波处理时间70 s、浸提温度80℃、浸提时间为30 min,这种工艺可使黄酮提取率达到最好。

With ginger as raw material,ethanol extracting with microwave processing to distill flavonoids from ginger with single factor test and orthogral test,the optimum extracting condition of flavonoids from ginger,such as microwave power,microwave radiation heating time,extraction temperature,extraction time,etc.,were discussed. Experimental results showed that the optimum conditions of ethanol extracting with microwave processing were as follows： 200 W of microwave power for 70 s,extracting at 80 ℃ for 30 min. Using this technology,the extraction rate of total flavonoids in ginger achieved the best.