西兰花种子中萝卜硫素酶解浸提工艺研究

Leaching Process Research of Sulforaphane with Broccoli Seeds

西兰花中含有丰富的抗癌物质,对大多数癌症都有较强的作用。本文研究了从西兰花种子中酶解浸提萝卜硫素的工艺,确定其最佳的酶解浸提参数。通过单因素梯度实验研究,采用自身芥子苷酶酶解以及密封浸提方式提取西兰花种子中萝卜硫素,主要对酶解时间,酶解料液比,酶解p H,超声浸提乙醇浓度,超声浸提时间,超声浸提次数几种浸提参数进行研究。结果表明:西兰花种子中萝卜硫素酶解浸提最佳条件为:酶解时间为8 h以上,酶解料液比为1∶2,酶解p H为7,超声浸提溶剂为80%乙醇,超声浸提时间为30 min,超声浸提的次数为2次。西兰花种子中萝卜硫素的酶解浸提工艺参数达到最佳时,其提取率可以大大的提高。

Broccoli contains abundant anti- cancer substances,for most cancer it has a stronger effect. This experiment studied Enzyme solution leaching process of sulforaphane in broccoli seeds,determining the optimum enzymic extraction parameters. Through the single factor gradient experiment research,using own mustard glucoside enzyme digestion and seal extraction method to extract sulforaphane in broccoli seeds,enzymatic hydrolysis time,solid- liquid ratio,acidity leaching,extraction of ethanol concentration,extraction time and ultrasonic extraction times were mainly studied. Results showed that the optimum leaching conditions were as follows： enzymolysis time of 8 h,solid- liquid ratio of 1∶2,leaching p H of 7,extraction solvent of 80% ethanol,extraction time for 30 min,the number of ultrasonic extraction of 2 times. The extraction parameter of sulforaphane gets to the best,its extraction efficiency can be greatly improved.