横山水库春季浮游生物RAPD-DNA指纹与物种组成关系分析

Analysis of relationship between DNA fingerprinting by RAPD and species of spring plankton in Hengshan reservoir

2013年4月,对浙江横山水库4个站位12个采样点的浮游生物群落进行了DNA多态性的RAPD指纹分析和种类组成鉴定,并通过聚类分析探讨了DNA指纹拓扑结构与物种组成对应关系。结果表明：筛选出的12条随机引物共获得87条长度在120~1 700 bp的谱带,多态率为87.4%;各站位平均有56.2条谱带,其中1号站位下层最多为63条,4号站位中层最少为50条;共观察到64种浮游生物,其中4号站位的上层采样点种类最多为27种,3号站位上层最少为16种,其它各采样点在17~25种不等,12个采样点均出现的种类有曲壳藻、卷曲纤维藻、小球藻、细胶鞘藻和简壳虫。基于物种组成与RAPD的系统聚类分析,可以将12个采样点的浮游生物群落划分为两大类。表明横山水库春季浮游生物群落DNA指纹结构与物种组成密切相关。

The RAPD fingerprinting based on the DNA polymorphism of the plankton communities at 12 sampling points from 4 stations in Hengshan Reservoir,Zhejiang,was analyzed and the species were identified in April 2013. The relationship between topological structure of DNA fingerprinting and species was discussed by the cluster analysis. The results showed that 87 bands with the length ranging from 120 to 1700 bp were gained from 12 screened random primers,and the polymorphic rate was 87. 4%. On average,56. 2bands were observed from each station. A maximum number of 63 bands were observed from the lower level of station 1 and a minimum of 50 bands were observed from the middle level of station 4. Sixty-four kinds of planktons were identified from 4 stations with the maximum number of 27 in the upper level of station 4 and the minimum of 16 in the upper level of station 3. 17- 25 kinds of planktons were observed from other stations. Achnanthes sp.,Ankistrodesmus convolutus,Chlorella vulgaris,Phormidium tenue and Tintinnidium sp. were all detected from 12 sampling points. The plankton communities from 12 sampling points can be divided into two types by systematic cluster analysis based on the species and RAPD. This study reveals a close relationship between the DNA fingerprinting structure of the plankton community and species in Hengshan Reservoir.