染料木黄酮诱导前列腺癌DU-145细胞凋亡的研究

Study of genistein on inducing apoptosis in prostate cancer DU-145 cells

目的探讨染料木黄酮诱导前列腺癌细胞系DU-145细胞凋亡的作用。方法在Caspase抑制剂Z-VAD存在或不存在的情况下,以染料木黄酮作用于前列腺癌DU-145细胞,采用MTT法检测细胞存活率、Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡、PI染色法流式细胞术检测细胞周期、JC-1染色法流式细胞术测定细胞线粒体膜电位(△ψm),比色检测试剂盒测定caspase-8活性。结果染料木黄酮能明显降低前列腺癌DU-145细胞的存活率、诱导癌细胞凋亡、降低△ψm及提高caspase-8活性(全部P<0.05)。Z-VAD能够逆转Gen抗前列腺癌的效应。结论染料木黄酮使细胞阻滞于G0和G1,分别通过凋亡信号通路的外源性和内源性途径,诱导前列腺癌细胞凋亡。

Objective To investigate the effects of genistein.（Gen）.on the apoptosis of human prostate cancer DU-145 cells. Methods Human prostate cancer cell line DU-145 cells were treated with Gen with or without pan-caspase inhibitor Z-VAD（Z-VAD-FMK）. Cell viability was examined by MTT assay..Apoptosis was determined using Annexin V-FITC / PI staining and flow cytometry..Mitochondrial membrane potential.（△ψm）.was analyzed by JC-1 staining and flow cytometry.Caspase-8 activity was measured by colorimetric assay kits. Results Gen inhibited viability, induced apoptosis,.decreased △ψm and increased caspase-8 activity.（All P values were less than 0.05） in DU-145 cells. In addition, its effects could be reversed by Z-VAD. Conclusion Gen can induce apoptosis in human prostate cancer DU-145 cells by activation of both intrinsic and extrinsic apoptotic pathways.