抑癌基因PTEN与Beclin1在膀胱癌中的表达及临床意义

Expression of PTEN and Beclin1 in bladder cancer patients

目的探讨抑癌基因PTEN与Beclin1在膀胱癌组织中的表达及临床意义。方法收集65例膀胱癌患者癌组织及癌旁组织,15例正常膀胱组织,采用免疫组织化学方法检测组织PTEN与Beclin1表达,Spearman相关分析癌组织中两者的相关性。结果PTEN在癌组织,癌旁组织及正常组织中的高表达率分别为53.8%,81.5%和93.3%。Beclin1在癌组织,癌旁组织及正常组织中的高表达率分别为58.5%,84.6%和86.7%。PTEN与Beclin1在癌组织的高表达率显著高于癌旁组织及正常组织(P均>0.05)。癌组织中Beclin1和PTEN表达与患者组织学分级及临床分期相关,而与患者性别及年龄无关。癌组织Beclin1和PTEN高表达率随着患者组织学分级和临床分期增高均呈趋势下降(P均<0.05)。癌组织中Beclin1表达与PTEN表达呈正相关(P<0.001)。结论Beclin1和PTEN可能与膀胱癌的发生发展有关。

Objective To investigate the expression of PTEN and Beclin1 in bladder cancer patients and to evaluate its clinical significance. Methods Immunohistochemical method was used to determine the expression of PTEN and Beclin1 in 65 cases of bladder cancer patients and the para-carcinoma tissues, and another 15 cases of normal bladder tissues. The relationship between PTEN and Beclin1 in cancer tissue was analyzed by Spearman correlation analysis. Results PTEN expression rate in cancer tissues,adjacent tissues and normal tissues were 53.8%,81.5% and 93.3%, respectively.Beclin1 high expression rate in cancer tissues,adjacent tissues and normal tissues were 58.5%, 84.6%and 86.7%, respectively. PTEN and Beclin1 high expression rate in cancer tissues were significantly higher than that of adjacent tissue and normal tissue（all P values were more than 0.05）. PTEN and Beclin1 high expression rate in cancer tissues were correlated with histological grade and clinical staging of patients, regardless of patient gender and age. Beclin1 and PTEN expression decreased along with histological grade and clinical staging of patients increased, respectively（all P values were less than 0.05）. Beclin1 expression was positively correlated with PTEN expression in cancer tissues（P〈0.001）. Conclusion The expression of PTEN and Beclin1 in bladder cancer were associated with tumor occurrence, progression.