冬虫夏草不同部位来源的菌株及多次传代固体发酵特性

The solid fermentation of Ophiocordyceps sinensis strains isolated from different parts of specimen and different generations

从西藏新鲜冬虫夏草不同部位分离菌株,并进行固体培养,比较菌丝生长、分生孢子产生情况及不同传代次数菌株的固体培养特性,以获得产生分生孢子数量最多的菌株用于蝙蝠蛾幼虫的侵染。结果表明:单子囊孢子、双子囊孢子和多子囊孢子菌株在斜面上出草能力较好,固体发酵时菌丝生长旺盛,分生孢子产生较多,显著高于组织分离菌株;组织分离菌株中,子座来源的菌株在谷壳和泥炭土培养基中,其产孢能力高于虫体来源的菌株;多次传代(5次传代)菌株在固体斜面上有较明显的退化现象,但在液体及固体发酵方面有着较好的生长以及分生孢子产量。

Growth and conidium production of Ophiocordyceps sinensis in solid fermentation among the different strains isolated from different parts of specimen and different generations were compared. The results showed that strains generated from the ascospores had a better ability of sporulation than the strains isolated from sclerotium and stroma. After five generations, the strains grew well in liquid or solid fermentation, although they obviously degenerated in slant culture.